caveolin-1蛋白在骨折愈合过程中表达的实验研究  

Experimental Study on Expression of Caveolin- 1 during Fracture Healing

作　　者：肖峰[1] 刘光华[2] 刘邦忠[2] 益莉娜[1] 邹珏[1] 张金宇[1] 薛宴旻 王秀玲[1] 

机构地区：[1]上海市第七人民医院病理科,200137 [2]复旦大学附属中山医院康复科

出　　处：《医学研究杂志》2015年第9期90-93,共4页Journal of Medical Research

基　　金：上海市第七人民医院新星培养基金资助项目

摘　　要：目的观察骨折愈合不同时期骨折部位caveolin-1蛋白的表达规律。方法 S-D大鼠40只,建立骨缺损模型。于术后1、2、3、4周随机选取10只大鼠,测定骨折处骨痂骨密度值。制作石蜡切片,进行HE染色及caveolin-1蛋白免疫组化染色。结果随着骨折愈合时间延长,骨折部位骨痂骨密度逐渐增加,于术后第4周达最大。组织学结果显示,第1周,间充质细胞及软骨细胞膜上开始有caveolin-1蛋白表达;第2周,软骨细胞大量增殖,其膜上caveolin-1蛋白表达增加;第3周,软骨细胞退变、凋亡,其细胞膜上caveolin-1蛋白表达开始减少;第4周,软骨细胞被成骨细胞取代,caveolin-1蛋白表达基本消失。结论骨折后caveolin-1蛋白表达呈现先升后降的趋势,并与软骨细胞出现、消失有关,推测caveolin-1蛋白与软骨细胞分化、增殖有关,而与骨折后骨矿化过程无关。Objective To observe the expression of caveolin - 1 during fracture healing. Methods Forty male SD rats were enrolled. Animal model of cortical bone defect in right proximal tibia of rats were performed. Ten rats were randomly sacrificed at week 1,2, 3 ,and 4. The bone mineral density（BMD） of the callus at the fracture point was measured. Then the callus were cut down and sliced for histological and immunohistochemistry observation. Results The BMD examination showed that callus BMD increased with time flowed, and got a maximal figure at week 4. Histological examination showed that at the first week,expression of caveolin - 1 was detected on the plasma membrane of mesenchymal cell and cartilage cells. At the second week,with the proliferation of cartilage cells, expression of cave- olin - 1 increased. In week 3, expression of caveolin - 1 decreased while the cartilage cells apoptosised. In week 4, caveolin - 1 was almost disappeared while cartilage cells were replaced by osteoblasts. Conclusion Expression of caveolin - 1 increased at first and decreased afterwards during fracture healing. This phenomenon was accompanied by the appearance and vanishing of cartilage cells. So it is specula- ted that caveolin - 1 has a relationship with cartilage cells differentiation and proliferation, but has no relationship with the course of mineralization.

关 键 词：骨折愈合 小凹蛋白-1 骨密度 

分 类 号：R3[医药卫生—基础医学]