可溶性Jagged1对大鼠静脉桥狭窄的抑制作用  

Soluble Jagged1 Inhibits the Restenosis of Vein Graft in Rat

作　　者：龚丹[1] 毛志福[1] 肖永光[1] 

机构地区：[1]武汉大学人民医院胸外科,430060

出　　处：《医学研究杂志》2015年第9期125-127,共3页Journal of Medical Research

摘　　要：目的研究可溶性Jagged1对大鼠静脉桥狭窄的抑制作用。方法成年SD大鼠随机分为正常组、实验组和对照组,每组16只。转染sJag1到实验组SD大鼠中。实验组和对照组中将大鼠颈外静脉移植至颈总动脉建立静脉桥移植模型,而正常组颈外静脉原位吻合。分别于术后第7、14、21、28天收集移植静脉,行HE染色后分别观察中层厚度和PCNA阳性指数,用Western blot法测定sJag1、Notch1和SM MHC、SM22α蛋白的表达。结果对照组静脉桥明显狭窄,但实验组静脉桥狭窄明显被抑制。sJag1蛋白在实验组处于高表达状态。对照组Notch蛋白的表达明显增高,而实验组中s Jag1却能有效抑制Notch蛋白的表达。静脉桥移植后收缩型蛋白SM22和SM MHC表达明显降低,而s Jag1能够促使其表达回复。结论可溶性Jagged1可以有效抑制静脉桥狭窄的发生。Objective To discuss if soluble Jagged 1 （ sJagl ） inhibits the restenosis of vein graft in rat. Methods Forty - eight adult rats used in this study were divided into three groups（ n = 16 for each group） ,normal group, the experimental group and the control group. Nucleic acids with SJagl and transfection reagent were injected to rats in the experimental group from the caudal vein for three days. The autogenous jugular vein was grafted into the carotid artery by micro - surgery in experimental group and control group and into the jugular Vein in situ in normal group. After the operation,rats in each group were fed in the same way. At 7 days, 14 days, 21 days and 28 days after grafting, the grafted from four of each groups vessel were taken out. Middle thickness and PCNA positive index after HE stai- ning was observed. The expression of sJagl, Notchl and SM MHC, SM22et protein was measured by Western blot. Results Intimal hyperplasia（IH） and anti -proliferating cell nuclear antibody（PCNA） index of smooth muscle cell（SMC）of vein graft in experiment group were lower than those in control. The expression of SJagl protein in the experimental group was in a high state. Notch protein in control group was obviously increased, and sJagl can effectively restrain the expression of Notch protein. And the expression of SM MHC and SM22α protein in experiment group was higher than that of control group. Conclusion Soluble Jaggedl can significantly inhibit the restenosis of vein graft in rats.

关 键 词：可溶性Jagged1 静脉桥 血管平滑肌细胞 

分 类 号：R6[医药卫生—外科学]