中华绒螯蟹Myostatin基因在蜕皮过程中的表达分析  

Expression analysis of Myostatin during molting cycle in Eriocheir sinensis

作　　者：王伟[1,2] 吴旭干[1] 徐蕾[1] 姚琴琴[1] 成永旭[1,3] 

机构地区：[1]上海海洋大学水产种质资源发掘与利用教育部重点试验室,上海201306 [2]中国水产科学研究院东海水产研究所,上海200090 [3]上海海洋大学上海高校知识服务平台水产动物遗传育种中心,上海201306

出　　处：《上海海洋大学学报》2015年第5期662-667,共6页Journal of Shanghai Ocean University

基　　金：上海市科委科研计划项目(13231203504;13320502100);科技部科技型中小企业技术创新项目(14C26213201214);国家农业成果转化项目(2012GB2C000147);上海高校水产学一流学科建设项目(沪教科2012-62)

摘　　要：通过荧光定量PCR(qR T-PCR)技术对中华绒螯蟹(Eriocheir sinensis)Myostatin(EsM STN)基因在不同组织及蜕皮阶段的表达情况进行了分析。结果显示:(1)EsM STN在蜕皮间期(C期)中华绒螯蟹肌肉组织中的基因表达水平最高,胸神经节和脑神经节中也有较高水平的表达,暗示EsM STN基因的功能并不局限于肌肉生长的调节。(2)在不同蜕皮阶段,肌肉中EsM STN的基因表达水平在蜕皮后期(AB期)最高,蜕皮前期(D期)最低,推测EsM STN可能正向调节中华绒螯蟹肌肉生长,参与肌肉组织蜕皮后的生长以及蜕皮前的萎缩过程;肝胰腺、上皮组织中EsM STN的最高基因表达水平都出现蜕皮后的AB期,C期或D期相对表达量最低,这可能与上述器官的细胞增殖主要发生在AB期有关;胸神经节中EsM STN的表达水平在蜕皮期(E期)最高,D期最低,暗示EsM STN正向调节胸神经节中的神经发生。In present study,the expression differences of myostatin（ MSTN） in various tissues of Eriocheir sinensis during molting cycle were analyzed by q RT-PCR. The results showed that,（ 1） Es MSTN（ Eriocheir sinensis MSTN） had its highest expression level in muscle,while higher expression levels were also observed in thoracic ganglia and brain ganglia at stage C,suggesting its physiological function was not restricted to muscle.（ 2） Es MSTN was expressed the highest at AB and the lowest at D stage of molting cycle in muscle,suggesting that Es MSTN might be a positive growth regulator in E. sinensis,involved in the significant expansion and severe atrophy of the muscle at AB stage and D stage,respectively. Whereas in hepatopancreas and epithelium,Es MSTN m RNA expression levels were the highest at AB stage,and the lowest at C or D stage,due probably to cell proliferation at AB stage in these tissues. In addition,the peak value of Es MSTN m RNA in thoracic ganglia occurred at stage AB and its lowest expression level was observed at stage D,which implied that Es MSTN may regulate the nerogenesis in this tissue positively.

关 键 词：中华绒螯蟹 Myostatin(MSTN) 表达分析 蜕皮 

分 类 号：S917[农业科学—水产科学]