肼苯哒嗪联合硝酸异山梨醇酯对心力衰竭大鼠的影响  

Effects of hydralazine isosorbide dinitrate on heart failure of rats

作　　者：柴连海 王丽丽 陈文生[3] 

机构地区：[1]唐山市工人医院检验科,河北唐山063000 [2]唐山市工人医院心外一科,河北唐山063000 [3]唐山市工人医院心内科,河北唐山063000

出　　处：《中国临床药理学杂志》2015年第19期1946-1948,共3页The Chinese Journal of Clinical Pharmacology

基　　金：2013年唐山市科学技术研究与发展指导计划基金资助项目(13130269b)

摘　　要：目的探讨肼苯哒嗪联合硝酸异山梨醇酯对心力衰竭模型大鼠的影响。方法 40只成年健康雄性大鼠,随机选取10只为正常组,其余进行心力衰竭模型复制,将模型大鼠按数字表法随机分为3组,模型组、实验组、对照组,每组10只。模型组与正常组每日灌胃生理盐水3 m L,对照组予以贝那普利(90mg·kg-1)+比索洛尔氢氯噻嗪片(45 mg·kg-1)灌胃3 m L,实验组予以硝酸异山梨醇酯(800 mg·kg-1)+肼苯哒嗪(225 mg·kg-1),每天灌胃1次3 m L。实验结束后,比较各组大鼠心功能、半胱氨酸(Cys)、同型半胱氨酸(Hcy)、脑钠肽(BNP)、肿瘤坏死因子α(TNF-α)水平及心肌组织形态。结果与模型组比较,实验组与对照组大鼠的BNP、TNF-α水平水平较低,Cys及Hcy水平较高,心功能恢复较好;与对照组比较,实验组大鼠BNP、TNF-α水平水平较低,Cys及Hcy水平较高,心功能恢复较好(均P<0.05)。结论肼苯哒嗪联合硝酸异山梨醇酯能够明显缓解心力衰竭症状对机体的影响,降低血脑钠肽及改善左心室功能、抑制肿瘤坏死因子及同型半胱氨酸的表达。Objective To explore the effect of hydralazine isosorbide dinitrate on heart failure rats. Methods Forty adult healthy male rats were selected and 10 rats were selected randomly to be normal group,the rest were replicated heart failure model. The 30 rats were randomly divided into 3 groups after the succeed of replication. The model group and normal group were given normal saline 3 m L,the control group was given benner pury（ 90 mg·kg^-1） ＋ bisoprolol fumarate and hydrochlorothiazide tablets（ 45mg·kg^-1） intragastric administration of 3 m L. The experimental group received isosorbide dinitrate（ 800 mg·kg^-1） ＋ hydralazine（ 225mg·kg^-1）,daily irrigation stomach once a 3 m L. Heart function,cysteine,homocysteine,brain natriuretic peptide（ BNP）,tumor necrosis factor alpha（ TNF- α） and myocardial tissue morphology were compared after the treatment. Results Compared with the model group,the level of BNP,TNF- α of the experimental group and the control group were lower,cysteine and hemocyanin were higher,cardiac function recovered.Compared with the control group,BNP,TNF- α of the experimental group were lower,cysteine and hemocyanin were higher,cardiac function was better（ P〈0. 05）. Conclusion Hydralazine and isosorbide dinitrate can significantly alleviate the symptoms of heart failure on the body effect,reduce blood brain natriuretic peptide and improve left ventricular function,inhibit the expression of TNF- α and homocysteine.

关 键 词：肼苯哒嗪 硝酸异山梨醇酯 慢性心衰 肿瘤坏死因子 

分 类 号：R972.3[医药卫生—药品] R965.1[医药卫生—药学]