石斛兰叶片再生体系的建立  

Establishment of regeneration from leaves in Dendrobium spp.

作　　者：冯莹[1,2] 赖钟雄[2] 

机构地区：[1]泉州师范学院资源与环境学院,福建泉州362000 [2]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2015年第5期472-477,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家支撑计划资助项目(2007BAD07B03)

摘　　要：以石斛兰叶片为材料,建立了叶片外植体的离体再生体系.结果表明:KC+4 mg·L-16-BA+1 mg·L-1NAA是石斛兰叶片诱导原球茎的最佳培养基,诱导率为11.99%;原球茎适宜的增殖培养基为KC+2 mg·L-16-BA+0.5 mg·L-1NAA,增殖倍数达到8.02;分化培养基为1/2 MS+0.5 mg·L-16-BA;最佳的壮苗生根培养基为1/2 MS+1 mg·L-1NAA,最佳移栽基质为棕树皮,成活率达到93.33%.The establishment of the regeneration system of Dendrobium was conducted to induce protocorm-like bodies( PLBs) leaves. The results showed that the best medium to induce PLBs from leaves was KC+4 mg·L-16-BA+1 mg·L-1NAA,and the ratio was 11.99%. KC+2 mg·L-16-BA+0.5 mg·L-1NAA was the greatest growth way of PLBs with the proliferation times reaching 8.02. 1 / 2 MS+0.5 mg·L-16-BA was taken as the best differential medium. Meanwhile the fittest medium for root growth was 1 / 2 MS+1.0 mg·L-1NAA. The survival rate of plantlets was 93.33% on the palmaebark transferring medium.

关 键 词：石斛兰 原球茎 增殖 分化 小苗 

分 类 号：S682.31[农业科学—观赏园艺]