香蕉果实冷胁迫相关MaWRKY11转录因子的特性、互作蛋白筛选与鉴定  

Characterization and Interacting-protein Identification of MaWRKY11 Transcription Factor Related to Cold Stress from Banana Fruits

作　　者：徐群刚 邝健飞[1] 单伟[1] 陆旺金[1] 陈建业[1] 

机构地区：[1]华南农业大学园艺学院广东省果蔬保鲜重点实验室,广州510642

出　　处：《热带亚热带植物学报》2015年第5期543-552,共10页Journal of Tropical and Subtropical Botany

基　　金：广东省高等学校科技创新重点项目(2012CXZD001);国家自然科学基金项目(31172007);国家香蕉现代农业产业技术体系项目(CARS-32-09)资助

摘　　要：为探讨香蕉(Musa acuminata)响应冷胁迫的分子机制,从香蕉果实冷害的数字基因表达谱中筛选并分离了1个WRKY转录因子,命名为Ma WRKY11。Ma WRKY11具有2个WRKY保守结构域,属于I类WRKY成员,定位于细胞核,是核蛋白。Ma WRKY11具有转录激活活性,且激活区在N端。实时荧光定量PCR分析表明Ma WRKY11受冷胁迫诱导,外源茉莉酸甲酯(Me JA)处理减轻香蕉果实冷害的同时也上调了其表达。另外,酵母双杂交筛选表明,Ma WRKY11可与脱水诱导的早期应答蛋白Ma ERD相互作用。这些表明Ma WRKY11可能通过与逆境相关蛋白如Ma ERD互作来响应香蕉果实的冷胁迫。In order to understand the molecular mechanism, one WRKY transcription factor, named as Ma WRKY11, was isolated and identifi ed from Digital Gene Expression Data(DGE) of banana(Musa acuminata) fruits. The results showed that Ma WRKY11 contained two WRKY conserved domains, belonging to Group I of WRKY family, and located in nucleus as a nuclear protein. The Ma WRKY11 possessed transcriptional activity in yeast cells and the transcriptional domain located at its N-terminus. Moreover, real-time quantitative PCR displayed that Ma WRKY11 was induced by cold stress, and chilling injury of banana fruits reduced and the expression of Ma WRKY11 was up-regulated treated by Me JA. In addition, C-terminus of Ma WRKY11 was used as a bait to screen its potential interacting proteins by using yeast two-hybrid system, and it was found that Ma WRKY11 could interact with early responsive to dehydration protein, Ma ERD, which was also induced by cold. So, it was suggested that Ma WRKY11 might respond to chilling stress through interacting with stress-related proteins, such as Ma ERD.

关 键 词：香蕉 果实 WRKY转录因子 冷胁迫 互作蛋白 

分 类 号：S668.1[农业科学—果树学]