针刺太冲和天枢对腹泻型肠易激综合征大鼠5-羟色胺、去甲肾上腺素及降钙素基因相关肽的影响  

Effects of Electroacupuncture on “Taichong” and “Tianshu” on Levels of 5-HT, NE and CGRP in Rats with Diarrhea Type Irritable Bowel Syndrome

作　　者：卢岩[1] 孙英霞[1] 于晓华[1] 张晶[1] 孙钊[1] 李金玲[1] 张喜鹏[2] 王彤[3] 王世军[4] 

机构地区：[1]山东中医药大学针灸推拿学院,山东济南250355 [2]山东中医药大学基础医学院,山东济南250355 [3]山东中医药大学护理学院,山东济南250355 [4]山东中医药大学国际教育学院,山东济南250355

出　　处：《中国中医药信息杂志》2015年第10期50-53,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(2011CB505206);山东省自然科学基金(ZR2012HL12);山东中医药大学大学生研究训练计划项目(2014413)

摘　　要：目的探讨电针不同穴位对腹泻型肠易激综合征(D-IBS)模型大鼠的效应差异。方法采用急慢性应激刺激法制作D-IBS大鼠模型。将实验动物分为对照组、模型组、天枢+足三里组、太冲+足三里组,针刺组分别给予双侧相应穴位电针刺激20 min,隔日1次,共治疗7次。观察各组大鼠体征状况,检测各组大鼠血浆5-羟色胺(5-HT)、去甲肾上腺素(NE)、降钙素基因相关肽(CGRP)的含量。结果与对照组比较,模型组大鼠血浆5-HT、NE含量明显增加,CGRP含量降低(P<0.05);与模型组比较,针刺组大鼠5-HT、NE含量明显降低,CGRP含量升高(P<0.05);两针刺组间比较无明显差异。结论针刺"太冲"和"天枢"可能通过降低模型大鼠血浆5-HT、NE含量,增加CGRP含量达到治疗D-IBS的目的。Objective To discuss the effects of electroacupuncture intervention in different acupoints of rats models with diarrhea type irritable bowel syndrome(D-IBS). Methods The rat models were established with acute and chronic stimulation. The rats were divided into the normal control group, model group, 'Taichong'(LR3) + 'Zusanli'(ST36) group, and 'Tianshu'(ST25) + 'Zusanli'(ST36) group. Electroacupuncture was applied to bilateral corresponding acupoints for 20 minutes, every other day for 7 times. The physical signs of each rat were observed. The contents of 5-HT, NE and CGRP in the rats' plasma were detected by sandwich ELISA. Results Compared with the normal group, the contents of 5-HT and NE in the model group were obviously higher and the content of CGRP decreased(P <0.05). Compared with the model group, the contents of 5-HT and NE in all treatment groups were lower and the content of CGRP increased(P <0.05). There was no difference between the 'Taichong'(LR3) + 'Zusanli'(ST36) and 'Tianshu'(ST25) + 'Zusanli'(ST36) groups. Conclusion The mechanism of electroacupuncture may be realized through lowering the contents of 5-HT and NE in the model rats and increasing the content of CGRP to treat D-IBS.

关 键 词：腹泻型肠易激综合征 电针 5-羟色胺 去甲肾上腺素 降钙素基因相关肽 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]