左归降糖解郁方对糖尿病并发抑郁症大鼠海马谷氨酸及N-甲基-D-天冬氨酸受体2A、2B的影响  

Effects of Zuogui Jiangtang Jieyu Formula on Expressions of Glutamate, NR2A and NR2B in Hippocampus of Diabetic Rats with Depression

作　　者：王宇红[1,2] 谭小雯[2] 柴上 杨蕙[3] 曾嵘[2] 孟盼[1] 张秀丽[1] 韩远山[1] 

机构地区：[1]湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地,湖南长沙410007 [2]湖南中医药大学药学院,湖南长沙410007 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中医药信息杂志》2015年第10期70-73,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81373578;81403379);湖南省自然科学基金(13JJ5030);长沙市科技局重点项目(1106184-11);湖南省高校创新平台开放基金项目(13k074)

摘　　要：目的观察左归降糖解郁方对糖尿病并发抑郁症大鼠海马谷氨酸、N-甲基-D-天冬氨酸受体(NMDA receptor,NR)2A及NR2B表达的影响,探讨其对糖尿病并发抑郁症大鼠海马损伤的保护机制。方法建立糖尿病并发抑郁症大鼠模型,随机分为模型组、阳性药组及左归降糖解郁方高、中、低剂量组,以正常大鼠为正常组,每组16只,各给药组给予相应药物灌胃,连续28 d。采用Open-field实验评价大鼠行为变化,ELISA检测海马谷氨酸含量,免疫荧光法检测海马NR2A、NR2B表达。结果与正常组比较,模型组大鼠自主活动次数明显减少(P<0.01),海马谷氨酸含量明显升高(P<0.01),NR2A、NR2B表达明显升高(P<0.01);与模型组比较,阳性药组和左归降糖解郁方高剂量组大鼠自主活动次数明显增加(P<0.01),海马谷氨酸含量明显下降(P<0.01),NR2A、NR2B表达降低(P<0.05)。结论左归降糖解郁方可明显改善糖尿病并发抑郁症大鼠的抑郁行为,其药效作用可能与调控大鼠海马谷氨酸含量及NR2A、NR2B表达有关。Objective To observe the effect of Zuogui Jiangtang Jieyu Formula on the expressions of glutamate, NR2 A and NR2 B in hippocampus of diabetic rats with depression;To explore the mechanism of protective effect. Methods Diabetes with depression rat models were established and then were randomly divided into the model group, positive medicine group, high-, mediumand low-dose Zuogui Jiangtang Jieyu Formula groups. Normal rats acted as normal group, 16 rats per group. After 28 days of administration, Open-field test was used to detect the behavior of the rats;glutamate content of hippocampus was detected by ELISA;the expressions of NR2 A and NR2 B in rat hippocampus were detected by immunofluorescence. Results Compared with normal group, automatic activity times of rats in model group decreased significantly(P <0.01);both glutamate content(P <0.01) and expressions of NR2 A, NR2B(P <0.01) increased significantly. Compared with the model group, automatic activity times of rats in positive medicine group and high-dose Zuogui Jiangtang Jieyu Formula group significantly increased(P <0.01);glutamate content dropped(P <0.01);expressions of NR2 A and NR2 B decreased(P <0.05). Conclusion Zuogui Jiangtang Jieyu Formula can improve depressive behavior of diabetic rats with depression, which may be related to the regulation of glutamate content and expressions of NR2 A and NR2 B in hippocampus.

关 键 词：左归降糖解郁方 糖尿病 抑郁症 谷氨酸 N-甲基-D-天冬氨酸受体2A N-甲基-D-天冬氨酸受体2B 大鼠 

分 类 号：R285.5[医药卫生—中药学]