秦皮总香豆素不同提取工艺对比研究  

Comparative Study on Cortex Fraxini Total Coumarin of Different Extraction Processes

作　　者：王瑞海[1] 柏冬[1] 刘寨华[1] 包强[2] 叶迎[1] 刘丽梅[1] 

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700 [2]甘肃省中医院,甘肃兰州730050

出　　处：《中国中医药信息杂志》2015年第10期86-90,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：中国中医科学院基本科研业务费自主选题项目(YZ-1411)

摘　　要：目的考察不同提取工艺对秦皮总香豆素提取率及主要香豆素活性成分含量的影响,以确定其适宜的提取工艺。方法采用正交设计法,以综合指标秦皮总香豆素提取率为考察指标,分别以水和醇作为溶剂,对秦皮提取工艺进行优选。结果秦皮水提取最佳工艺:秦皮饮片加9倍量水,煎煮3次,每次煎煮1.5 h。水提取秦皮出膏率为28.87%,总香豆素含量为19.26%、提取率为5.56%,秦皮甲素、秦皮乙素、秦皮苷、秦皮素总含量为13.47%。秦皮醇提取最佳工艺:秦皮饮片用8倍量75%乙醇回流2次,每次2 h。醇提取秦皮出膏率为30.47%,总香豆素含量为21.72%、提取率为6.62%,秦皮甲素、秦皮乙素、秦皮苷、秦皮素总含量为15.29%。2种工艺对比,醇提取比水提取出膏率提高了5.54%,总香豆素含量提高了12.77%、提取率提高了19.06%,4种香豆素成分总含量提高了13.51%,差异均有统计学意义(P<0.05)。结论醇提取工艺各考察指标均优于水提取工艺,故应采用75%乙醇提取秦皮总香豆素,且工艺稳定。Objective To study the effects of different extraction processes on extraction rate of cortex fraxini total coumarin and active constitute percentage of major coumarin;To establish a proper extraction process. Methods Orthogonal design method was applied to set comprehensive index cortex fraxini total coumarin extraction rate as inspecting index. Water and alcohol were used as solvent respectively to optimize the extraction process of cortex fraxini. Results Optimal water extraction process:cortex fraxini decoction pieces mixed with nine times of water, decocted for three times, 90 mins each time. The pasty fluid generating rate of cortex fraxini was 28.87%, total coumarin percentage was 19.26%, extraction rate was 5.56%, total percentage of Aesculin, Aesculetin, Fraxin, Fraxetin was 13.47%, when water was used as solvent. Optimal alcohol extraction process:cortex fraxini decoction pieces mixed with eight times of 75% ethyl alcohol, refluxed twice, two hours each time. The pasty fluid generating rate of cortex fraxini was 30.47%, total coumarin percentage was 21.72%, extraction rate was 6.62%, total percentage of Aesculin, Aesculetin, Fraxin, Fraxetin was 15.29%, when alcohol was used as solvent. It was found that using alcohol as solvent had a 5.54% higher pasty fluid generating rate, a 12.77% higher total coumarion percentage, a 19.06% higher total coumarin extraction rate, and a 13.51% higher percentage of total four coumarin constitutes than using water, with statistical significance. Conclusion Extraction process by using alcohol as solvent is better than using water. So the optimal and stable extraction process of cortex fraxini total coumarin is using 75% alcohol as solvent.

关 键 词：秦皮 提取工艺 总香豆素 提取率 

分 类 号：R284.2[医药卫生—中药学]