RP-HPLC测定藏药六味木香丸中木香烃内酯和去氢木香内酯含量  

RP-HPLC Determination of Costunolide and Dehydrocostus Lactone in Tibetan Medicine Liuwei Muxiang Pill

作　　者：焦正花[1] 刘效栓[1] 杨金草 

机构地区：[1]甘肃省中医院药学部,甘肃兰州730050 [2]甘南州食品药品检验所,甘肃甘南747000

出　　处：《中国中医药信息杂志》2015年第10期94-96,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的 建立同时测定藏药六味木香丸中木香烃内酯和去氢木香内酯含量的分析方法.方法 采用高效液相色谱法,色谱柱为XDB-C18 柱（4.6 mm×50 mm,1.8 μm）,以甲醇-水（60∶40）为流动相,流速0.8 mL/min,检测波长225 nm.结果 藏药六味木香丸中木香烃内酯、去氢木香内酯的线性范围分别为0.235 5～1.413 μg、0.291 3～1.748 μg,平均加样回收率分别为99.25%、99.41%.结论 该方法准确可靠,结果稳定,重复性好,为评价藏药六味木香丸的质量提供了依据.Objective To establish the method to analyze the contents of Costunolide and Dehydrocostus Lactone in Tibetan medicine Liuwei Muxiang Pill. Methods HPLC method was used;the XDB-C18 column(4.6 mm × 50 mm, 1.8 μm) was adopted;the mobile phase was methanolwater(60∶40);the flow rate was 0.8 m L/min;UV detection was 225 nm. Results The linear range of Costunolide and Dehydrocostus Lactone was 0.235 5-1.413 μg and 0.291 3-1.748 μg. The average recoveries were 99.25% and 99.41%, respectively. Conclusion The method is accurate, reliable, stable and with good repeatability, which provides the basis for the quality evaluation of Tibetan medicine Liuwei Muxiang Pill.

关 键 词：藏药六味木香丸 木香烃内酯 去氢木香内酯 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]