归芪多糖对早衰WI-38细胞端粒酶活性的影响  

Effects of Guiqi Polysaccharide on Telomerase Activity in Premature Senescence WI-38 Cells

作　　者：蒲秀瑛[1] 于双[1] 樊文博[1] 任菁[1] 刘璐[1] 马小龙[1] 张伟杰[1] 

机构地区：[1]兰州理工大学生命科学与工程学院甘肃省中藏药筛选评价及深加工重点实验室,甘肃兰州730050

出　　处：《中国中医药信息杂志》2015年第11期50-53,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81260070);兰州市科技局项目(2011-1-71)

摘　　要：目的观察归芪多糖对过氧化氢(H2O2)诱导的WI-38早衰细胞端粒酶活性的影响,并探讨其作用机制。方法 H2O2诱导建立早衰WI-38细胞模型,MTT法检测不同浓度H2O2对WI-38细胞活力的影响,筛选出用于诱导细胞早衰的H2O2浓度。实验细胞分为正常组、模型组和归芪多糖组,化学染色法和ELISA检测归芪多糖对衰老细胞β-半乳糖苷酶和端粒酶活性的影响。结果 200μmol/L H2O2可诱导18代WI-38细胞出现早衰。归芪多糖干预后早衰细胞β-半乳糖苷酶和端粒酶活性均未呈现出衰老样变化,与模型组比较差异有统计学意义(P<0.01),与正常组比较差异无统计学意义(P>0.05)。结论归芪多糖可明显增强衰老细胞的端粒酶活性并显著抑制衰老相关β-半乳糖苷酶活性。Objective To investigate the effects of Guiqi polysaccharide(GQP) on the telomerase activity in premature senescence of WI-38 cells;To discuss its mechanism of action. Methods MTT assay was used to observe the effects of different concentrations of H2O2 on the proliferation of WI-38 cells and screened the best concentration of H2O2 to induce premature senescence cellular model in WI-38 cells. ELISA and chemical staining method were used to evaluate the protection of GQP in telomerase activity and senescence-associated β-galactosidase activity in premature cellular senescence. Results 200 μmol/L H2O2 treatment could induce premature senescence in WI-38 cells. There were no significant differences in senescence-associated β-galactosidase and telomerase activity between GQP group and normal control group(P >0.05), but had significant difference with model group(P <0.01). Conclusion GQP can increase the telomerase activity and inhibit the senescence-associated β-galactosidase activity in premature senescence of WI-38 cells.

关 键 词：归芪多糖 过氧化氢 WI-38细胞 Β-半乳糖苷酶 端粒酶 

分 类 号：R285.5[医药卫生—中药学]