脊髓康对脊髓损伤大鼠血清脑源性神经营养因子及神经生长因子表达的影响  

Effects of Jisuikang on Expressions of BDNF and NGF in Serum of Rats with Spinal Cord Injury

作　　者：郭杨[1] 马勇[1,2] 董维[1] 潘娅岚 黄桂成[1] 颜睿杰 

机构地区：[1]南京中医药大学骨伤研究所,江苏南京210023 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《中国中医药信息杂志》2015年第11期65-68,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：江苏省普通高校研究生科研创新计划项目(CXZZ12_0609);江苏省自然科学基金(BK2011180);江苏省高等学校大学生创新创业训练计划项目(201410315078X)

摘　　要：目的观察脊髓康对脊髓损伤大鼠血清脑源性神经营养因子(BDNF)及神经生长因子(NGF)蛋白表达的影响,探讨其改善损伤轴突再生微环境和抗脊髓损伤的作用机制。方法 90只SD大鼠采用改良Allen's法建立大鼠急性脊髓损伤模型,随机抽取15只作为假手术组,其余75只造模成功后随机分为模型组、强的松组及脊髓康高、中、低剂量组。各治疗组予相应药物灌胃,假手术组和模型组予等体积生理盐水灌胃。记录期间各组大鼠活动及死亡情况,于干预后3、7、14 d处死大鼠,颈动脉采血,ELISA检测血清BDNF、NGF表达。结果模型组大鼠脊髓损伤后均出现典型的截瘫综合征。术后3 d,BDNF表达模型组与假手术组比较差异无统计学意义(P>0.05),而各治疗组均高于假手术组(P<0.05,P<0.01);术后7、14 d,各治疗组和模型组均高于假手术组(P<0.01),其中强的松组、脊髓康中剂量组高于模型组(P<0.05,P<0.01)。各治疗组和模型组NGF表达均高于假手术组,术后3、7 d各治疗组明显高于模型组;术后14 d与模型组比较,强的松组和脊髓康中、低剂量组仍维持较高水平(P<0.05,P<0.01)。结论脊髓康能有效促进脊髓损伤大鼠血清BDNF、NGF表达,并在14 d内维持较高水平,从而改善轴突再生微环境,促进神经再生。Objective To clarify the effects of Jisuikang on expression levels of BDNF and NGF protein in serum of rats with spinal cord injury;To explore its probable mechanism to improve the axon regeneration microenvironment and resistance spinal cord injury. Methods Ninety SD rats were chosen to establish acute spinal cord injury model through modified Allen's method. 15 rats were chosen randomly as sham-operation group, and the other 75 rats were randomly divided into model group, prednisone group and high-, medium-, and low-dose Jisuikang groups. All administration groups were given relevant medicine for gavage, while rats in sham-operation group and model group were given normal saline with the same volume for gavage. The activity and death condition of rats were recorded. Rats were put to death on the 3rd, 7th and 14 th d of intervention for carotid artery blood collection. The expressions of BDNF and NGF in serum were detected by ELISA. Results After modeling, the rats in model group showed a typical paraplegia syndrome. 3 day after operation, BDNF showed no statistical significance between model group and prednisone group, while each treatment group showed significant difference when compared with prednisone group(P <0.05, P <0.01). 7 d and 14 d after operation, compared with the modelgroup, BDNF of prednisone group and medium-dose Jisuikang group showed statistical significance, and there was no significant difference between prednisone group and medium-dose Jisuikang group(P <0.05, P <0.01). The expression of NGF in each treatment group at each time point with the prednisone group showed statistical significance. 3 d and 7 d after operation, among prednisone group, high-and medium-dose Jisuikang groups, the expression of NGF was significantly higher than model group. 14 d after operation, compared with the model group, the expression of NGF in medium-and low-dose Jisuikang groups and prednisone group still maintained at a relatively high level, with statistical significance(P <0.05, P <0.01). Conclusion Jisuikang

关 键 词：脊髓康 脊髓损伤 脑源性神经营养因子 神经生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]