白疕合剂对体外培养人永生化角质形成细胞分泌血管内皮生长因子及其受体2表达的影响  

Effects of White Mange Mixture on VEGF Content and Genetic and Protein Expressions of VEGFR-2 in Cultured HaCaT Cells

作　　者：陈兴[1] 李忻红[1] 卢益萍[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中国中医药信息杂志》2015年第11期73-75,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：辽宁省自然科学基金(201102149)

摘　　要：目的观察中药白疕合剂干预人永生化角质形成细胞(HaC aT细胞)后,对其血管内皮生长因子(VEGF)的分泌量及其受体2(VEGFR-2)基因和蛋白表达的影响,并探讨其作用机制。方法体外培养HaC aT细胞建立银屑病实验模型。SD大鼠随机分为空白血清组、阿维A组和白疕合剂低、中、高剂量组,并单设空白对照组。给药后制备血清,干预HaC aT细胞模型。ELISA检测VEGF分泌量,RT-PCR检测VEGFR-2基因表达,Western blot检测VEGFR-2蛋白表达。结果与空白对照组比较,白疕合剂低、中、高剂量组干预HaC a T细胞后,VEGF分泌量及VEGFR-2基因和蛋白表达均明显抑制,且呈浓度依赖性。结论白疕合剂可能通过抑制VEGF分泌量、降低VEGFR-2基因和蛋白表达以达到治疗银屑病的作用。Objective To observe the effects of White Mange Mixture on the content of VEGF and the genetic and protein expressions of VEGFR-2 of HaCaT cells;To explore its mechanism. Methods HaCaT cells were cultured in vitro to establish psoriasis experimental model. SD rats were randomly divided into the blank serum group, white mange mixture of low-, medium-and highdose groups and acitretin group, meanwhile to set up blank control group. Cultured HaCaT cells model were intervened with different medicine serum. ELISA was applied to detect the content of VEGF. RT-PCR and Western blot were used to detect the genetic and protein expressions of VEGFR-2. Results Compared with the blank control group, White Mange Mixture of low-, mediumand high-dose groups interventing HaCaT cells could obviously inhibit the secretion of VEGF and decrease the genetic and protein expression of VEGFR-2 in a dose-dependent manner. Conclusion White Mange Mixture plays a role in the treatment of psoriasis possibly through inhibiting the content of VEGF and decreasing the genetic and protein expressions of VEGFR-2.

关 键 词：白疕合剂 人永生化角质形成细胞 血管内皮生长因子 血管内皮生长因子受体-2 大鼠 

分 类 号：R285.5[医药卫生—中药学]