安石榴苷在Caco-2细胞的摄取特性研究  

Uptake Property Study on Punicalagin in Caco-2 Cells

作　　者：谢莉[1] 田莉[1,2] 高晓黎[3] 

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830011 [2]新疆名医名方与特色方剂学重点实验室,新疆乌鲁木齐830011 [3]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《中国中医药信息杂志》2015年第11期79-82,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81260668)

摘　　要：目的 研究安石榴苷在Caco-2 细胞的摄取特性.方法 采用Caco-2 细胞单层模型研究安石榴苷在不同时间、浓度、pH 值、温度、P-糖蛋白抑制剂条件下对细胞摄取的影响.采用高效液相色谱法测定安石榴苷在细胞中的含量.结果 安石榴苷在0.024 8～0.992 μg 范围内呈良好线性关系（r =0.997 9）.安石榴苷在Caco-2 细胞上的摄取呈现-定的时间、浓度和pH 值依赖性,且P-糖蛋白抑制剂能显著增加安石榴苷的摄取量.结论 该方法操作简单、灵敏,安石榴苷在Caco-2 细胞上的摄取主要是被动转运,P-糖蛋白参与安石榴苷的转运过程.Objective To study the uptake property of punicalagin in Caco-2 cells. Methods Caco-2 cell monolayer model was used to research the effects of punicalagin on cell uptake in different times, concentrations, p H values, temperatures and P-gp inhibitors. HPLC was used to determine the content of punicalagin in cells. Results The linearity of punicalagin concentration curve was found in the range of 0.024 8-0.992 μg(r =0.997 9). Uptake determination of punicalagin in Caco-2 cells absorbed a certain amount of time, concentration and p H dependence and P-gp inhibitors could significantly increase the punicalagin uptake. Conclusion This method is simple and sensitive to determine the punicalagin concentration in Caco-2 cells, which is mainly passively transported. P-gp is involved in punicalagin transport process.

关 键 词：安石榴苷 CACO-2细胞 摄取 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]