INS-1E细胞葡萄糖毒性模型的建立  

Establish Glucose Toxicity Model of INS-1E Cell

作　　者：丁静云 应颖[2] 邬磊[1] 伍茵[3] 孔祥臣[2] 曾庆[2] 梁真[1] 

机构地区：[1]安徽医科大学深圳二院临床学院老年科,广东深圳518036 [2]深圳大学糖尿病研究所,广东深圳518060 [3]安徽医科大学深圳二院临床学院内分泌科,广东深圳518036

出　　处：《现代生物医学进展》2015年第22期4244-4247,共4页Progress in Modern Biomedicine

基　　金：深圳市科技计划重点项目(201101006);深圳市科技研发资金基础研究项目(JC201105180813A)

摘　　要：目的:建立胰岛细胞系INS-1E细胞的葡萄糖毒性模型。方法:将INS-1E细胞分别在不同葡萄糖浓度(5.5 mmol/L、16.7mmol/L、25 mmol/L、30 mmol/L)的1640完全培养基中培养不同时间(48 h、72 h、96 h、120 h),分别在不同时间点取细胞进行细胞功能检测,实时荧光定量PCR法检测胰岛素m RNA的表达,ELISA检测葡萄糖刺激的胰岛素的分泌。结果:与对照组相比,高糖浓度(5.5 mmol/L、16.7 mmol/L、25 mmol/L、30 mmol/L)培养基中培养48 h后,INS-1E细胞的胰岛素合成和分泌的功能均增加(P均<0.05),随着培养基中葡萄糖浓度的升高以及培养时间的延长,INS-1E细胞胰岛素合成及分泌的功能逐渐下降,当在葡萄糖浓度为30 mmol/L的培养基中培养120 h后,胰岛素m RNA合成及葡萄糖刺激的胰岛素分泌均显著降低(P均<0.01)。结论:INS-1E细胞在30 m M的葡萄糖中培养120 h形成稳定的葡萄糖毒性模型。Objective： To establish glucose toxicity model of 1NS-1E cell. Methods： INS-1E cells were cultured in different glucose concentration （5.5 mmol/L, 16.7 mmol/L, 25 mmol/L and 30 mmol/L） 1640 fully culture medium in different time （48 h, 72 h, 96 h, 120 h）. Cells were taken at different time points to detect cell function. Insulin mRNA was detected by fluorescent quantitative PCR. Insulin secretion was tested by ELISA. Results： Compared with the normal glucose control group, after 48 h cultured in high glucose concentrations （5.5 mmol/L, 16.7 mmol / L, 25 mmol / L, 30 mmol / L）, insulin synthesis and secretion of 1NS-1E cells were increased （P〈0.05）. With increasing concentration of glucose in the medium and longer incubation time, insulin synthesis and secretion function of INS-1E cells was gradually declined. When cells were cultured 120 h in the medium with 30 mmol/L glucose concentration, insulin mRNA synthesis and glucose-stimulated insulin secretion were significantly reduced （P〈0.01）. Conclusions： Glucose toxicity model can be formed after 1NS-1E cell cultured 120 h in 30 mM glucose.

关 键 词：INS-1E细胞 葡萄糖毒性 胰岛素 

分 类 号：R587.1[医药卫生—内分泌] Q813[医药卫生—内科学]