人生长分化因子15单克隆抗体制备、鉴定及药用价值初探  

Preparation, Identification and Medicinal Value of Monoclonal Antibody against Growth Differentiation Factor-15 of Human

作　　者：郑蕾[1] 冯英明[1] 宋扬[1] 李红[1] 聂娜[1] 苏海川[1] 

机构地区：[1]第四军医大学唐都医院肿瘤科,陕西西安710038

出　　处：《现代生物医学进展》2015年第24期4601-4604,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81372255)

摘　　要：目的：制备稳定分泌抗人生长分化因子15（GDF15）单克隆抗体（m Ab）的杂交瘤细胞系,并对其分泌的m Ab进行鉴定。方法：根据人GDF15氨基酸序列特征,设计合成了8条能够免疫产生GDF15特异性抗体的抗原多肽,与VLP载体偶联后,免疫雌性BALB/c小鼠,利用杂交瘤技术制备鼠源抗人GDF15的m Ab,用间接ELISA检测m Ab腹水效价。结果：获得针对7个抗原多肽的12株稳定分泌抗人GDF15的杂交瘤细胞系,腹水m Ab效价可达1×104~1×109。结论：获得了针对不同抗原多肽的抗人GDF15的特异性m Ab,为进一步研发以GDF15为靶点的单克隆抗体抗肿瘤药物奠定了基础。Objective： To establish the specific hybridoma cell lines that secreted stably monoclonal antibody（m Ab） against growth differentiation factor-15（GDF15） from human. Methods： Eight antigen peptides which can produce GDF15 specific antibodies were designed and coupled with VLP carrier. Then female BALB/c mice were immunized with coupled VLP carrier and m Ab against GDF15 was prepared with hybridoma cell fusion technique. Indirect enzyme-linked immunosorben assary（ELISA） was used to evaluate the ascites titers of hybridoma cell lines. Results： Twelve hybridoma cell lines which can stable produce anti-GDF15 m Ab against seven antigen peptides were obtained. The titers of m Ab in ascites were ranged from 1×104to 1×109. Conclusions： The specific m Ab against different antigen peptides of GDF15 were produced, which will be significant for monoclonal antibody research of GDF15-targeted drugs.

关 键 词：人GDF15 杂交瘤 单克隆抗体 

分 类 号：Q95-3[生物学—动物学] Q78