尿酸性肾病中IL-1β、IRAK-4的表达及意义  

Expression and Significance of IL-1β, IRAK-4 in Uric Acid Nephropathy

作　　者：赵会文[1] 刘崇恺[2] 付正菊[1] 周蓓蓓[1] 李招霞 许士璐 

机构地区：[1]青岛大学附属医学内分泌科,山东青岛266021 [2]即墨市人民医院,山东青岛266200

出　　处：《现代生物医学进展》2015年第24期4645-4648,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81070686);市南区科技发展资金项目(201414027YY)

摘　　要：目的：通过研究尿酸性肾病动物模型中白介素-1（IL-1）β和白介素-1受体相关激酶4（IRAK-4）表达的意义,了解IL-1β信号通路在尿酸性肾病中的作用。方法：Wistar大鼠54只随机分为高尿酸血症组30只、正常组24只,制备尿酸性肾病大鼠模型,检测尿酸（UA）、尿素氮（BUN）、肌酐（CR）及肌酐清除率（Ccr）、24 h尿微量白蛋白（m A1b）;取肾脏组织行HE染色,观察形态学变化;免疫组化测定IL-1β的表达;荧光定量PCR检测IRAK-4 m RNA的水平。结果：高尿酸组2、4、6周时IL-1β的表达均增加,免疫组化评分（IHS）均明显升高（P〈0.01）;高尿酸血症组较正常组IRAK-4 m RNA在2、4、6周时均出现表达上调,4~6周IRAK-4 m RNA表达明显增加,与正常组比较有显著性差异（P〈0.01）。结论：IL-1β、IRAK-4参与了尿酸性肾病炎症反应的过程,可能为尿酸性肾病治疗提供新的可能。Objective： To study the expression and effect of interleukin 1（IL-1） β and interleukin-1 receptor-associated kinase 4（IRNK-4） in Uric Acid Nephropathy Rats, and investigate the significance of IL-1 β Signaling Pathway in the development of uric acid nephropathy. Methods： 54 Wistar rats were randomly divided into hyperuricemia group and normal control group. Establishing the rats model group to examine the levels of uric acid（UA）, serum urea nitrogen（BUN）, serum creatinin（CR） and 24 h urinary microalbumin（m A1b） in each group; Morphological changes were observed by HE staining; The expression levels of L-1β in renal tissue were detected by immunohistochemical method; The expression levels of IRAK-4 were detected by fluorescent quantitation PCR. Results： The Expression level of IL-1β was significantly higher in the hyperuricemia group after 2, 4, 6 weeks（P〈0.01）; IRAK-4 m RNA in renal tissue were increased in hyperuricemia group after 2,4,6 weeks, and significantly higher in 4-6 weeks（P〈0.01）. Conclusion： The expression of IL-1βand IRAK-4 may be associated with the progress of inflammation in uric acid nephropathy, which will provide a new option for treatment of this disease.

关 键 词：尿酸性肾病 白介素-1Β 白介素-1受体相关激酶4 

分 类 号：R589[医药卫生—内分泌]