严重烧伤对大鼠海马神经细胞自噬和神经胶质细胞反应的诱导作用  

Induction of severe burn on autophagy of nerve cells and astrocyte reaction in hippocampus tissue of rats

作　　者：李雷[1] 王永杰[1] 陈浩[1] 秦永刚[1] 周煜博[1] 赵嘉勋 郭丽[1] 

机构地区：[1]吉林大学公共卫生学院卫生毒理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2015年第5期898-901,I0001,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30972153;C180103);吉林省科技厅科技发展计划项目资助课题(20130413023GH)

摘　　要：目的:观察严重烧伤诱发的大鼠大脑海马体神经细胞的改变,并探讨其可能的作用机制。方法:将20只成年Wistar大鼠随机分为正常对照组和模型组,每组10只。模型组大鼠用于制备烧伤模型。在造模结束1周内,观察2组大鼠的饮食、饮水等情况;HE和尼氏体染色观察大鼠海马体神经细胞病理组织形态学;免疫组织化学法检测海马神经胶质细胞标志物神经胶质纤维酸性蛋白(GFAP)、神经元标志物微管相关蛋白2(MAP-2)、自噬标志物轻链3蛋白(LC3B)和Beclin 1的表达。结果:正常对照组大鼠进食、饮水正常;模型组大鼠烧伤后,短期内进食、饮水减少,萎靡少动。HE和尼氏体染色检测,正常对照组大鼠海马体无明显神经细胞损伤;模型组大鼠海马神经细胞水肿,核固缩,尼氏体溶解、紊乱。免疫组织化学法检测,模型组大鼠海马体神经细胞中GFAP、LC3B和Beclin 1表达水平明显高于正常对照组(P<0.05),MAP-2的表达水平明显低于正常对照组(P<0.05)。结论:大鼠严重烧伤可致海马神经细胞损伤,诱发神经胶质细胞反应,并启动神经细胞的过度自噬。Objective To observe the changes of nerve cells in hippocampus tissue of severe burn rats,and to discuss its possible mechanism.Methods 20 adult Wistar rats were randomly divided into normal control group（n=10）and model group（n=10）.The rats in model group were used to establish the severe burn models.1week after modeling,the eating and drinking state of rats in two groups were observed.The pathohistology of nerve cells in hippocampus tissue of rats were observed by HE and Nissl staining;the expressions of glial fibrillary acidic protein（GFAP）,microtubule-associated protein-2（MAP-2）,light chain 3B（LC3B）and Beclin 1were detected by immunohistochemistry method.Results The rats in normal control group ate and drank regularly,but the rats in model group were listless and inactive after burning.HE and Nissl staining results showed that the disappearance and disorder of Nissl bodies were observed in model group,and the nerve cells in hippocampus tissue of severe burn rats partly damaged with cellular swelling and nuclear pyknosis.There was no obvious damage of the nerve cells in normal control group.The immunohistochemistry results showed that the expression levels of GFAP,LC3 B,and Beclin 1in the rats in model group were significantly higher than those in normal control group（P〈0.05）,and the expression level of MAP-2was significantly lower than that in normal control group（P〈0.05）.Conclusion After severe burn,the hippocampus nerve cells of rats are damaged,which may result in astrocyte reaction and the increasing of nerve cells autophagy.

关 键 词：烧伤 海马 神经细胞损伤 神经胶质细胞反应 自噬 

分 类 号：R995[医药卫生—毒理学]