血必净对大鼠脑缺血再灌注损伤的保护作用及其机制  

Protective effect of Xuebijing on cerebral ischemia reperfusion injury in rats and its mechanism

作　　者：苏建华[1] 陈玉芳[2] 丁新生[3] 唐金荣[3] 肖杭[4] 林伟[1] 

机构地区：[1]江苏大学附属金坛医院神经内科,江苏金坛213200 [2]江苏省常州市第四人民医院病理科,江苏常州213000 [3]南京医科大学第一附属医院神经内科,江苏南京210029 [4]南京医科大学应用神经毒理研究所,江苏南京210029

出　　处：《吉林大学学报（医学版）》2015年第5期951-955,I0003,共6页Journal of Jilin University：Medicine Edition

基　　金：江苏省委人才办第四期"333工程"科研项目资助课题(BRA2012050);江苏省常州市科技局科研项目资助课题(WZ201043);江苏大学医学临床科技发展基金资助课题(JLY2010053)

摘　　要：目的：研究血必净对大鼠脑缺血再灌注损伤后神经细胞凋亡和细胞色素C（Cyt C）蛋白表达的影响,并探讨其作用机制。方法：将108只Wistar大鼠随机分为假手术组、模型组、尼莫地平组和血必净组,每组27只。采用线栓法栓塞大脑中动脉3h制作大鼠脑缺血再灌注模型;分别于6、12和24h采用TUNEL法检测大鼠大脑皮层神经细胞凋亡数;Western blotting法检测大脑皮层中Cyt C蛋白相对表达水平。结果：TUNEL法检测,与模型组比较,尼莫地平组和血必净组各时间点大鼠神经细胞凋亡数明显减少（P〈0.01）;与尼莫地平组比较,24h时血必净组大鼠神经细胞凋亡数减少（P〈0.05）。Western blotting法检测,6-24h模型组大鼠大脑皮层中Cyt C蛋白相对表达水平较假手术组升高（P〈0.05或P〈0.01）;6-24h尼莫地平组和血必净组大鼠大脑皮层中Cyt C蛋白相对表达水平较模型组明显降低（P〈0.01）;24h时血必净组大鼠大脑皮层中Cyt C蛋白相对表达水平较尼莫地平组降低（P〈0.01）。结论：血必净对脑缺血再灌注损伤神经细胞有抗凋亡作用,其抗凋亡效应与抑制Cyt C蛋白表达有关联。Objective To observe the effect of Xuebijing on the expression of cytochrome C（Cyt C）protein and apoptosis of neurons in the rats with ischemia reperfusion injury,and to explore the mechanism.Methods 108 Waster rats were randomly divided into sham operation group,model group,nimodipine group,and Xuebijing group（n=27）.The rat models of cerebral ischemia reperfusion were established by middle cerebral artery occlusion for 3hwith suture method.The apoptosis of neurons in perifocal cortex was detected by TUNEL method and the relative expression level of Cyt C protein was analyzed by Western blotting method at 6,12and24 h.Results The FUNEL results showed that compared with model group,the number of apoptotic neurons in nimodipine group and Xuebijing group was decreased obviously（P〈0.01）at each time point,and there was significant difference between Xuebijing group and nimodipine group at 24h（P〈0.05）.The Western blotting results showed that compared with sham operation group,the relative expression levels of Cyt C of the rats in model group were increased obviously at 6,12 and 24h（P〈0.05 or P〈0.01）.The relative expression level of Cyt C protein of the rats in nimodipine group and Xuebijing group were reduced significantly at every time point compared with model group（P〈0.01）;the relative expression level of Cyt C protein of the rats in Xuebijing group was lower than that in nimodipine group at 24h（P〈0.01）.Conclusion Xuebijing can inhibit the apoptosis after ischemia reperfusion injury,and the mechanism of anti-apoptosis may be related to the down-regulation of the expression of Cyt C protein.

关 键 词：血必净 脑缺血再灌注 细胞凋亡 细胞色素C 大鼠 Wistar 

分 类 号：R972[医药卫生—药品] R961[医药卫生—药学]