Biodegradation of nicosulfuron by a novel Alcaligenes faecalis strain ZWS11  

作　　者：Weisong Zhao Chen Wang Li Xu Chunqing Zhao Hongwu Liang Lihong Qiu 

机构地区：[1]College of Science, China Agricultural University

出　　处：《Journal of Environmental Sciences》2015年第9期151-162,共12页环境科学学报（英文版）

基　　金：supported by the National Basic Research Programme of China (No. 2010CB126104)

摘　　要：A bacterial strain ZWS11 was isolated from sulfonylurea herbicide-contaminated farmland soil and identified as a potential nicosulfuron-degrading bacterium. Based on morphological and physicochemical characterization of the bacterium and phylogenetic analysis of the 16 S r RNA sequence, strain ZWS11 was identified as Alcaligenes faecalis. The effects of the initial concentration of nicosulfuron, inoculation volume, and medium p H on degradation of nicosulfuron were investigated. Strain ZWS11 could degrade 80.56% of the initial nicosulfuron supplemented at 500.0 mg/L under the conditions of p H 7.0, 180 r/min and30°C after incubation for 6 days. Strain ZWS11 was also capable of degrading rimsulfuron,tribenuron-methyl and thifensulfuron-methyl. Four metabolites from biodegradation of nicosulfuron were identified, which were 2-aminosulfonyl-N, N-dimethylnicotinamide（M1）, 4, 6-dihydroxypyrimidine（M2）, 2-amino-4, 6-dimethoxypyrimidine（M3） and2-（1-（4,6-dimethoxy-pyrimidin-2-yl）-ureido）-N,N-dimethyl-nicotinamide（M4）. Among the metabolites detected, M2 was reported for the first time. Possible biodegradation pathways of nicosulfuron by strain ZWS11 were proposed. The degradation proceeded mainly via cleavage of the sulfonylurea bridge, O-dealkylation, and contraction of the sulfonylurea bridge by elimination of a sulfur dioxide group. The results provide valuable information for degradation of nicosulfuron in contaminated environments.A bacterial strain ZWS11 was isolated from sulfonylurea herbicide-contaminated farmland soil and identified as a potential nicosulfuron-degrading bacterium. Based on morphological and physicochemical characterization of the bacterium and phylogenetic analysis of the 16 S r RNA sequence, strain ZWS11 was identified as Alcaligenes faecalis. The effects of the initial concentration of nicosulfuron, inoculation volume, and medium p H on degradation of nicosulfuron were investigated. Strain ZWS11 could degrade 80.56% of the initial nicosulfuron supplemented at 500.0 mg/L under the conditions of p H 7.0, 180 r/min and30°C after incubation for 6 days. Strain ZWS11 was also capable of degrading rimsulfuron,tribenuron-methyl and thifensulfuron-methyl. Four metabolites from biodegradation of nicosulfuron were identified, which were 2-aminosulfonyl-N, N-dimethylnicotinamide（M1）, 4, 6-dihydroxypyrimidine（M2）, 2-amino-4, 6-dimethoxypyrimidine（M3） and2-（1-（4,6-dimethoxy-pyrimidin-2-yl）-ureido）-N,N-dimethyl-nicotinamide（M4）. Among the metabolites detected, M2 was reported for the first time. Possible biodegradation pathways of nicosulfuron by strain ZWS11 were proposed. The degradation proceeded mainly via cleavage of the sulfonylurea bridge, O-dealkylation, and contraction of the sulfonylurea bridge by elimination of a sulfur dioxide group. The results provide valuable information for degradation of nicosulfuron in contaminated environments.

关 键 词：Biodegradation Nicosulfuron Alcaligenes faecalis Metabolite 

分 类 号：X592[环境科学与工程—环境工程] X172