Postembryonic Development of Electrophysiological Characteristic of Primordial Hippocampus in Bufo bufo gargarizans  

中华大蟾蜍原始海马神经元电生理学特性的胚后发育（英文）

作　　者：王维[1] 邓昕旸 梁传成[1] 王勇[2] 王丽文[1] 

机构地区：[1]沈阳师范大学化学与生命科学学院,辽宁沈阳110034 [2]沈阳师范大学实验中心,辽宁沈阳110034

出　　处：《Agricultural Science & Technology》2015年第9期1978-1980,2024,共4页农业科学与技术（英文版）

基　　金：Supported by grants from the Liaoning Provincial Department of Education(2011196)~~

摘　　要：[Objective] The aim was to probe into developing changes of spontaneous electrical activities of primordial hippocampus in Bufo bufo gargarizans in postembryonic phase. [Method] Microelectrode technique was used to record extracellularly spontaneous electrical activities of neurons in primordial hippocampus of B.bufo gargarizans in postembryonic phase. [Result] Single, consecutive single, burst, consecutive burst and anomalous spontaneous firing were recorded in primordial hippocampus, in which four kinds of spontaneous firings were recorded in forepart of postembryonic development except the anomalous spontaneous firing, and the burst spontaneous firing and consecutive burst spontaneous firing were predominant. The five kinds of spontaneous firings mentioned were recorded in anaphase of postembryonic development and the single and burst spontaneous firing were predominant.Following with the development of primordial hippocampus, the amplitudes of action potential of single firing and consecutive firing enhanced gradually, and it reaches the maximum in the metamorphosis phase; the differences of the frequencies of consecutive single firing and consecutive burst firing were not significant. [Conclusion] Following with the development of primordial hippocampus, the neuronal electrical activities showed diversification, the developmental degree of the primordial hippocampus in the metamorphosis phase was close to the subadult and adult.[目的]探讨中华大蟾蜍(Bufo bufo gargarizans)原始海马神经元自发电活电生理学特性的胚后发育变化。[方法]应用微电极电生理技术,对中华大蟾蜍胚后原始海马神经元的自发放电活动进行在体胞外记录。[结果]原始海马神经元的自发放电分为单个放电、连续单个放电、簇状放电、连续簇状放电和不规则放电五种形式。胚后发育的早期为4种,以簇状放电和连续单个放电为主,没有记录到不规则放电;胚后发育的后期为5种,以单个和簇状放电为主。单个放电和连续放电的幅度随着发育的进行而增大,在变态期达最大;连续单个放电和连续簇状放电频率在变态期、亚成体期和成体期相近,差异不显著。[结论]随着原始海马的发育,神经元电活动形式逐渐呈现多样化,变态期原始海马的发育程度接近亚成体、成体。

关 键 词：Bufo bufo gargarizans Primordial hippocampus Spontaneous electrical activities Postembryonic phase 

分 类 号：Q424[生物学—神经生物学]