Screening of Saprolegniasis Preventive Drugs for Golden Trout (Oncorhynchus mykiss) Fertilized Eggs During Hatching  

金鳟受精卵孵化期间水霉病防治药物的筛选（英文）

作　　者：李绍戊[1] 刘红柏[1] 

机构地区：[1]中国水产科学研究院/黑龙江水产研究所,黑龙江哈尔滨150070

出　　处：《Agricultural Science & Technology》2015年第9期1981-1984,1988,共5页农业科学与技术（英文版）

基　　金：Supported by the Twelfth Five-year Plan"Integration and Demonstration of Key Technology of Raising Fish in Heilongjiang"(2012BAD25B10)~~

摘　　要：In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of two kinds of self-made Chinese herbal compounds（AB） and two kinds of chemical bactericides（peroxyacetic acid andhydrogen peroxide, CD） with three disinfection concentrations were compared in this study. The fertilized eggs of golden trout were randomly divided into 13 groups, each group with two parallels of 1 000 eggs. Drugs at high,medium and low concentration were respectively used to immerse the fertilized eggs35 min for disinfection once a day. The numbers of live and dead eggs were recorded each day before disinfection and the numbers of survival eyed eggs were counted in each experimental group after finishing the whole test. The results indicated that all of four kinds of drugs used here had a certain effect on prevention and control of saprolegniasis during hatching of fertilized eggs of golden trout, and also showed a correlation between the concentrations and effects. After the selection of several drugs, we found that group B-medium, C-medium and D-high exhibited the most remarkable effects on control of saprolegniasis. However, careful consideration should be given to chemical drugs residues and their potential hazard to the eyed eggs. Therefore, Chinese herbal compound B was suggested to be used for prevention and control of saprolegniasis to achieve the healthy aquaculture. And the concentration and operation time of Chinese herbal compound B should be strictly controlled when used in disinfection of fertilized eggs.为了筛选金鳟受精卵孵化期间水霉感染的有效防治药物,比较研究了2种自组中药复方(A和B)及2种化学除菌剂(过氧乙酸和过氧化氢,C和D)3种消毒浓度下的作用效果。将金鳟发眼卵随机分为13组,每组2个平行,每个平行1 000粒卵,选取适当的高、中、低浓度对鱼卵进行浸泡消毒,每天1次,每次持续35 min,每日消毒前进行活卵、死卵(霉菌感染死亡及其他原因死亡)计数,实验结束后,对每组存活发眼卵进行计数。结果表明:本实验所选用的2种自组中药复方和2种化药除菌剂对金鳟受精卵孵化期间水霉病的防治均有一定效果,且表现出一定的浓度相关性。经筛选,B中剂量组、C中剂量组和D高剂量组效果最为显著,但是考虑化药后期残留及对发眼卵的潜在危害,建议选择中药复方B进行金鳟受精卵水霉病防治,以进一步实现健康绿色养殖。选用中药复方B进行消毒时,应严格保证其消毒浓度及作用时间,以确保较好的消毒效果。

关 键 词：Golden trout（Oncorhynchus mykiss） Fertilized eggs SAPROLEGNIASIS Preventive drugs 

分 类 号：S943[农业科学—水产养殖]