壳聚糖絮凝法精制川牛膝总多糖酶提取液的工艺研究  

Study on the Flocculating Purification Process of Total Polysaccharides from Cyathula Officinalis Kuan by Using Chitosan

作　　者：成悦[1] 刘傲霞[1] 夏玉婷[1] 谷旭晗[1] 韩伟[1] 

机构地区：[1]华东理工大学中药现代化工程中心,上海200237

出　　处：《机电信息》2015年第29期31-36,共6页

基　　金：国家大学生创新创业训练计划;项目编号:131025159;华东理工大学本科教育教学改革项目(2013年)

摘　　要：以壳聚糖为絮凝剂对川牛膝的总多糖进行了絮凝纯化研究。通过单因素考察及正交试验得到优化的工艺条件为:絮凝温度45℃、壳聚糖用量0.4 m L/g生药量、絮凝时间2 h、药液浓缩比1:12、药液p H=4。此时,多糖保留率76.32%、脱蛋白率64.25%,与传统醇沉工艺相比,絮凝法得到的多糖保留率和纯度较高。The essay was aimed at studying flocculating purification of total polysaccharides by using chitosan. The optimum conditions were obtained through single factor and orthogonal experiment. The results were as follows：the temperature was 45 ℃ , dosage of chitosan was 0.4 mL/g raw material, flocculation time was 2 h, solution concentration ratio was 1：12, pH of solution was 4. Under this condition, polysaccharides retention rate was 76.32%, deproteinizing rate of 64.25%. Compared with the traditional alcohol precipitation process, the retention rate and purity of the polysaccharide of the flocculation purification process are higher.

关 键 词：壳聚糖 絮凝 川牛膝 总多糖 试剂 

分 类 号：TQ460.6[化学工程—制药化工]