PT和APTT检测常见影响因素分析  

Study on common influencing factors for detecting PT and APTT

作　　者：朱名超[1] 朱娅[2] 孙莉[3] 

机构地区：[1]天门市第一人民医院检验科,湖北天门431700 [2]天门市第一人民医院NICU科,湖北天门431700 [3]襄阳职业技术学院医学院,湖北襄阳441021

出　　处：《临床和实验医学杂志》2015年第19期1653-1655,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨血浆凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）检测的常见影响因素,从而提出合理化建议,提高检验质量。方法选择不同年龄组、同一标本不同放置时间、不同离心时间及血液与抗凝剂的不同比例分别测定PT、APTT值。结果 〈1岁组与〉18岁成人组比较PT、APTT明显延长,差异均有显著性（P〈0.01）,1~5岁PT与成人无差异,但APTT延长（P〈0.01）;同一标本室温放置4 h以上PT延长,放置6 h及6 h以上PT、APTT均延长;与抗凝剂与血液比例=1︰9相比,〈1︰9和〉1︰9 PT、APTT均明显延长（P均〈0.01）;不同离心时间对PT、APTT检测结果比较差异均无显著性（P〉0.05）。结论应建立〈1岁组PT、APTT的生物参考区间;PT测定应在4 h以内,APTT不宜超过6 h;当标本量大时,可适当减少离心时间;采集标本时要严格控制血液与抗凝剂比例。Objective To explore the common influencing factors for detecting prothrombin time（PT）and acti - vated partial thrombo-plastin time（APTT）in order to provide reasonable advice and to improve the quality of detection. Methods The detection of PT and APTT in different age groups and same sample with different placed time as well as different centrifugal time and different proportion of blood and anticoagu-lant respectively had been carried out. Results The difference in PT and APTT between 1 year old age group and adult group had obvious statisti-cal significance（ P ﹤ 0. 01）. There was no difference in PT between 1 ~ 5 years old children group and adult group,but APTT was prolonged（ P ﹤ 0. 01）. The PT was longer when the samples put in room temperature over 4 hours,both PT and APTT were longer when the duration for placement of samples was 6 hours and more. When the proportion of anticoagulant and blood had been over or under 1：9,the results of PT and APTT showed significant difference with statistical significance（ P ﹤ 0. 01）. Different centrifugal time had no statistical significance on PT and APTT detecting results（ P ﹥ 0. 05）. Conclusion The reference intervals about PT and APTT and the duration for detection in 1 year old age group should be limited within 4 hours when detecting PT,as for APTT,the duration should be limited within 6 hours. If the amount of sample is large,the centrifugal time can be appropriately reduced,and the proportion of blood and anticoagulant in samples should be strictly controlled.

关 键 词：凝血酶原时间 活化部分凝血活酶时间 影响因素 

分 类 号：R446.11[医药卫生—诊断学]