彭泽鲫F_1和F_2代雌、雄鱼激素含量及芳香化酶活力差异研究  

Analysis on the difference of steroid hormone levels and aromatase activities between male and female Pengze crucian carp in F_1 and F_2 offspring

作　　者：郑尧[1,2,3] 张永泉[1] 陈家长[2] 邴旭文[2] 尹家胜[1] 王在照[3] 

机构地区：[1]农业部淡水水产生物技术与遗传育种重点实验室/中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]中国水产科学研究院淡水渔业研究中心/农业部长江下游渔业资源环境科学观测实验站/中国水产科学研究院内陆渔业生态环境和资源重点开放实验室,江苏无锡214081 [3]西北农林科技大学动物科技学院/陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《南京农业大学学报》2015年第5期864-868,共5页Journal of Nanjing Agricultural University

基　　金：农业部淡水水产生物技术与遗传育种重点实验室开放课题(FBB2015-04);国家自然科学基金项目(31270547);现代农业产业技术体系建设专项(CARS-49);国家科技支撑计划项目(2015BAD13B03)

摘　　要：[目的]本文旨在比较实验室和池塘养殖F1代,高、低密度实验室养殖F2代雌、雄鱼在激素含量和芳香化酶活力上的差异。[方法]采用ELISA方法对激素含量和芳香化酶活力进行了检测。[结果]发现实验室养殖F1代雌鱼全鱼组织雌二醇(E2)含量极显著高于雄鱼(P<0.01)。池塘养殖F1代雌鱼脑中E2和睾酮(T)含量均极显著高于雄鱼,且卵巢中的芳香化酶活力极显著低于雄鱼(P<0.01)。低密度养殖组F2代中卵巢T含量极显著低于高密度养殖组精巢中的含量(P<0.01)。低密度养殖组F2代雌鱼脑、卵巢中的E2含量分别极显著高于高密度养殖组F2代雄鱼脑、精巢中的含量(P<0.01)。F2代雌鱼脑中的芳香化酶活力极显著低于雄鱼中的,但卵巢中的芳香化酶活力极显著高于精巢中的(P<0.01)。[结论]实验室和池塘养殖F1代,高、低密度实验室养殖F2代雌、雄鱼激素含量和芳香化酶活力存在差异。[Objectives]The purpose of this study was to find the difference of steroid hormone levels and aromatase activities between different genders in laboratory and pond culture condition（ F1offspring）,together with the same situation between high-density and low density laboratory culture groups（ F2offspring）. [Methods]The present study used ELISA to detect steroid hormone levels and aromatase activities. [Results]The whole body 17β-estradiol（ E2） levels in females were extremely significantly higher than the males of F1 offsprings under laboratory culture condition（ P0.01）. Female brain E2 and testosterone（ T） were extremely significantly higher than the males under pond culture condition（ P0.01）. Aromatase activity in ovary was extremely significantly lower than testes（ P0.01）. However in F2 offsprings,ovarian T in low-density group was extremely significantly lower than high-density group,while brain and ovarian E2 contents in low-density group were strongly significantly higher than high-density group respectively（ P 0. 01）.Aromatase activities in female brains were highly significant lower than males,while aromatase activities in ovaries were strongly significantly higher than testes. [Conclusions]The present study presented the difference of steroid hormone levels and aromatase activities between different genders of F1,F2 offspring.

关 键 词：雌核发育 彭泽鲫 睾酮 雌二醇 芳香化酶 

分 类 号：S965.117[农业科学—水产养殖]