高效液相色谱法对肉鸡组织样品中黄曲霉毒素B1残留量的测定  

Determination of the residual quantity of aflatoxin B1 in the samples from broiler tissues using high performance liquid chromatography

作　　者：姚婉[1] 秦顺义[1] 王昕 赵方红[1] 刘丽娜[1] 张兰秀 

机构地区：[1]天津农学院动物科学与动物医学学院,天津300384 [2]河北省盐山县畜牧水产局,河北盐山061300

出　　处：《黑龙江畜牧兽医》2015年第10期281-283,287,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31101867);天津市高校"优秀青年教师资助计划"项目

摘　　要：为了检测肉鸡组织中黄曲霉毒素B1（AFBl）的残留量，试验采用柱前衍生一高效液相色谱（HPLC）的分析方法，并周该方法对样品进行检测。结果表明：AFB1在0．5～50．0μg／L范围内具有良好的线性关系，相关系数大于0．999，检出限为0．1μg/kg,信噪比（S／N）=3：1。在0．5μg／kg、1．0μg/kg、2．0μg／kg3个添加水平下肝脏组织平均回收率为91．0％-107．0％，肌肉组织平均回收率为94．0％-105．4％，相对标准偏差（RSD）分别为0．8％～1．7％、1．2％-2．O％。说明本方法具有灵敏性、准确性和可重复性，可对动物组织样中AFBl残留进行定性和定量检测。To detect residual quantity of aflatoxin B1 （AFB1） in broiler tissues, the method of a high performance liquid chromatography （ HPLC ） with pre - column derivatization was used to detect the samples from broiler tissues. The results showed that the calibration curves of AFB1 had good linearity relationship in the range from 0.5 to 50.0 μg/L, and the correlation coefficient was greater than 0. 999. The limits of detection were 0.1 μg/kg with a signal to noise ratio of 3：1. The average recovery rates of AFB1 in liver tissue were from 91.0% to 107.0% at three adding levels of O. 5 μg/kg, 1.0 μg/kg and 2.0 μg/kg for AFB1, while the average recovery rates of AFB1 in muscle tissue were from 94.0% to 105.4%, and the values of relative standard deviation （RSD） for the average recovery rates of AFB1 in liver tissue and muscle tissue were from 0.8% to 1.7% and from 1.2% to 2.0% , respectively. The results indicate that the method is suitable for qualitative and quantita- tive detection of aflatoxin B1 residues in the samples from animal tissues due to its sensitivity, accuracy and repeatability.

关 键 词：肉鸡组织样品 柱前衍生-高效液相色谱(HPLC) 黄曲霉毒素B1(AFB1) 检出限 回收率 

分 类 号：S859.84[农业科学—临床兽医学]