高尿酸血症鹌鹑模型的氧化应激状态研究  

Study on oxidative stress condition of hyperuricemia quail model

作　　者：林志健[1] 张冰[1] 李丽玉[1] 朱春胜[1] 

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《临床和实验医学杂志》2015年第20期1665-1668,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(NO.81403152);教育部高等学校博士学科点专项科研基金(NO.20130013120001;20120013130002);北京中医药大学青年教师专项自主课题(2013-QNJSZX008);北京中医药大学科研创新团队(2011-CXTD-014)

摘　　要：目的观察高嘌呤饮食诱导的高尿酸血症鹌鹑模型体内氧化应激水平的变化。方法采用高嘌呤饮食诱导法建立高尿酸血症鹌鹑模型,通过检测尿酸代谢相关酶、磷酸戊糖途径及血清抗氧化能力,观察模型动物高血尿酸状态下的氧化应激情况。结果模型动物第14天、21天血清尿酸水平显著升高;第21天血清黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)、葡萄糖-6-磷酸脱氢酶(G6PD)活性显著升高;血清葡萄糖-6-磷酸(G6P)、还原型辅酶Ⅱ(NADPH)含量升高;血清过氧化物歧化酶(SOD)活性显著降低;血清谷胱甘肽过氧化物酶(GSH-PX)、谷胱甘肽还原酶(GSH-GR)活性及丙二醛(MDA)、还原型谷胱甘肽(GSH)含量显著升高。结论高嘌呤饮食诱导的高尿酸血症鹌鹑模型体内黄嘌呤氧化酶系统及磷酸戊糖途径活跃,抗氧化能力减弱,机体氧化应激水平升高。Objective To investigate the oxidative stress condition in hyperuricemia quail model. Methods Hyperuricemia quail model were induced by purine rich diet. Uric acid metabolic enzymes and pentose phosphate pathway were determined. Serum anti- oxidase agents were also determined. Results Serum uric acid level increased in model group on 14 th and 21 st day. The level of XOD,AOD,G6 PD,G6P,NADPH,GSH- PX,GSH- GR,MDA and GSH increased on 21 st day. Serum SOD activities were decreased significantly. Conclusion Purine rich diet can induce quail hyperuricemia. XOD and pentose phosphate pathway were activated in hyperuricemia quail model. Quails in model group were under oxidative stress condition,with anti- oxidative ability decreased.

关 键 词：鹌鹑模型 高尿酸血症 氧化应激 高嘌呤饮食 

分 类 号：R589.7[医药卫生—内分泌] R-332[医药卫生—内科学]