过氧化物酶增殖体激活受体β激动剂对急性肺损伤NF-κB信号通路的影响  

Influence of activator of peroxisome proliferator activated receptorβeffect on NF-κB signaling pathway of lung tissue of rats with acute lung injury

作　　者：朴光熙[1] 金红旭[1] 姜腾轩[1] 柳云恩[1] 张玉彪[1] 高燕[1] 

机构地区：[1]沈阳军区总医院急诊医学部全军重症(战)创伤救治中心实验室辽宁省重症创伤和器官保护重点实验室,沈阳110016

出　　处：《临床急诊杂志》2015年第9期678-681,共4页Journal of Clinical Emergency

基　　金：2012年辽宁省自然科学基金项目(No:201202243)

摘　　要：目的:研究过氧化物酶增殖体激活受体β(PPARβ)的激动剂对失血性休克诱导的急性肺损伤(ALI)大鼠肺组织NF-κB信号通路产生的影响。方法:复制失血性休克大鼠ALI模型,按完全随机原则将18只SD大鼠分为正常对照组(对照组)、失血性休克致ALI模型组(模型组)和PPARβ激动剂给药组(实验组)3组,每组6只大鼠。对照组不做任何处理;模型组先给大鼠静脉注射10%DMSO 3ml/kg,再复制失血性休克致ALI模型;实验组先给大鼠静脉注射PPARβ激动剂3ml/kg,再复制失血性休克致ALI模型。观察记录3组大鼠一般情况,观察动脉血气、肺湿/干(W/D)比值、肺组织病理改变,ELISA方法检测肺组织匀浆上清中IL-1和IL-10浓度水平,并使用Western blot方法检测肺组织NF-κB p65蛋白水平变化。结果:复制模型后1、2h,实验组大鼠PaO2高于模型组(P<0.05)。实验组和模型组大鼠W/D比值明显高于对照组(P<0.01),且实验组大鼠W/D比值明显低于模型组(P<0.01)。苏木精-伊红染色显示,对照组大鼠肺泡结构清晰,未见明显炎性细胞浸润和渗出;模型组大鼠肺泡腔内有大量炎性细胞浸润,可见微血栓及透明膜形成;实验组大鼠肺泡腔内有少量炎性细胞浸润,可见少量微血栓及透明膜形成。实验组和模型组大鼠肺组织匀浆中IL-1浓度水平均高于对照组(P<0.05),且实验组大鼠肺组织IL-1浓度水平低于模型组(P<0.05)。实验组大鼠肺组织匀浆中IL-10浓度水平高于对照组和模型组(P<0.05)。Western blot检测结果显示,模型组NF-κB p65蛋白相对表达量高于对照组(P<0.05),实验组NF-κB p65蛋白相对表达量低于模型组和对照组(P<0.05)。结论:PPARβ激动剂能明显抑制ALI后NF-κB的表达,抑制炎性反应,可能对ALI具有治疗价值。Objective：The influence of the activator of peroxisome proliferator-activated receptorβ（PPARβ）on NF-κB signaling pathway of lung tissue of rats suffering hemorrhagic shock-induced ALI is explored.Method：Eighteen rats were randomly divided into normal control group（control group）,hemorrhagic shock injury model group（model group）,and activator of peroxisome proliferator-activated receptorβ（PPARβ）group（experimental group）,each group consist of 6rats.Control group without any treatment,in terms of control group,inject 3ml/kg of 10% DMSO into the rats successively,and then replicate the hemorrhagic shock-induced ALI model of rats;in terms of Group C,inject 3ml/kg of activator of PPARβinto the rats successively,and then replicate the hemorrhagic shock-induced ALI model of rats.The arterial blood gas,wet/dry ratio and lung tissue pathology are observed and recorded;ELISA is adopted to determine the mass concentrations of IL-1and IL-10 in the homogenate and supernatants of lung tissue.Western blot method is used to observe the changes in NF-κB protein level of lung tissue.Result：The PaO2 of rats in the experimental group was higher than that in model group（P〈0.05）after replication model 1and 2h.The W/D ratio in the experimental group and the model group was significantly higher than that in the control group（P〈0.01）,and the W/D ratio in the experimental group was significantly lower than that in model group.In the experimental group and the model group,the levels of IL-1in lung tissue homogenate were higher than that in control group（P〈0.05）,and the level of IL-1in experimental group was lower than that in model group（P〈0.05）.In the experimental group,the level of IL-10 in lung tissue homogenate was higher than that in control group and model group（P〈0.05）.The results of Western blot indicate that the relative expression of NF-κB protein in the experimental group was lower than that in model group and control group（P〈0.05）,and the relative expression o

关 键 词：急性肺损伤 过氧化物酶增殖体激活受体β 炎症 NF-ΚB 

分 类 号：R563[医药卫生—呼吸系统]