舒血宁注射液对大鼠肝纤维化的防治作用和NF-κB/IκBα信号通路影响的研究  

Protective effect of Shuxuening injection on the expression of NF-κB/IκBα signaling pathway in rats with liver fibrosis

作　　者：周学东[1,2] 王蓉[1] 王媛媛[1] 原永芳[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院药剂科,上海201999 [2]上海市宝山区罗店医院药剂科,上海201908

出　　处：《中南药学》2015年第9期911-916,共6页Central South Pharmacy

基　　金：新华集团基金(No.14XJ22011);上海市宝山区科学技术委员会科研基金(No.14-E-5)

摘　　要：目的探讨舒血宁注射液对大鼠肝纤维化的防治作用及NF-κB/IκBα信号通路影响的研究。方法将SD大鼠随机分为正常对照组(简称对照组)、肝纤维化模型组(简称模型组)、舒血宁注射液给药组(简称给药组),每组12只。每周2次50%CCl4花生油溶液(1 m L·kg-1)灌胃6周建立大鼠肝纤维化模型。同时,给药组每日一次腹腔注射舒血宁注射液15 mg·kg-1。观察大鼠的生活状况、肝脏大体形态;HE染色检测大鼠肝脏病理学的变化;全自动生化分析仪检测血清谷丙转氨酶(ALT)及谷草转氨酶(AST);放射免疫法检测血清透明质酸(HA)、IV型胶原(CIV)、层黏蛋白(LN)和Ⅲ型前胶原肽(PⅢP);Western blot检测肝脏组织细胞核中NF-κB和细胞质中NF-κB、IκBα蛋白的表达。结果舒血宁注射液治疗后大鼠肝功能指标有明显下降,血清肝纤维化指标有显著降低,并且大鼠肝脏病理组织学结构有明显改善(P<0.05)。与模型组相比,舒血宁注射液能够上调肝组织细胞质中NF-κB和IκBα蛋白的表达,下调细胞核中NF-κB蛋白的表达(P<0.05)。结论舒血宁注射液可通过抑制NF-κB/IκBα信号通路发挥抗肝纤维化的作用。Objective To explore the protective effect of of Shuxuening injection on the expression of NF-κB/IκBαsignaling pathway in rats with liver fibrosis. Methods SD rats were randomly divided into a control group（n =12）, a model group（n = 12）, and a Shuxuening injection group（n = 12）. Liver fibrosis model was established by administering 50% CCl4（1 m L·kg- 1） twice a week. At the meantime, rats in the Shuxuening injection group were intraperitoneally injected Shuxuening 15 mg·kg- 1 once daily. The basic living condition of the rats and macroscopic features of the livers were observed. The pathological change of liver tissue was examined by HE staining. The serum levels of ALT and AST were analyzed by automated biochemistry analyzer. The serum levels of HA, CIV, LN and P Ⅲ P were determined by radioimmunoassay. The expressions of NF-κB and IκBα in the liver were determined by Western blot. Results Shuxuening injection group improved the pathologic manifestations and reduced the serum levels of ALT, AST, HA, CIV, LN and P Ⅲ P（P〈0.05）. The expression of NF-κB in the nucleolus was up-regulated and the expression of NF-κB and IκBα protein in the cytoplasm was down-regulated in the Shuxuening Injection group. Conclusion Shuxuening injection can prevent the progression of liver fibrosis possibly by inhibiting the expression of NF-κB/IκBα signaling pathway.

关 键 词：舒血宁注射液 银杏提取物 肝纤维化 NF-ΚB IΚBΑ 

分 类 号：R259[医药卫生—中西医结合]