PGDS和PGES在增生性瘢痕中的表达  

Expressions of PGDS and PGES in hyperplastic scar

作　　者：沈晓洁[1] 袁志坚[1] 周梅芳[1] 何文涓 沈惠亮 

机构地区：[1]无锡卫生高等职业技术学校,江苏省214028 [2]中国药科大学

出　　处：《江苏医药》2015年第18期2135-2137,共3页Jiangsu Medical Journal

基　　金：无锡市科技局科技计划项目(CSZ00N1234)

摘　　要：目的研究前列腺素D合成酶(PGDS)和前列腺素E合成酶(PGES)在增生性瘢痕组织中的表达。方法根据瘢痕形成时间,将47例患者分为烧伤早期组(A1组,9例)以及瘢痕形成后1个月(A2组,9例)、3个月(A3组,9例)、6个月(A4组,8例)、12个月(A5组,6例)和24个月组(A6组,6例);另设正常皮肤组织的对照组(B组,10例)。采用Western blot和免疫组化染色法检测各组PGDS、PGES蛋白表达。结果与B组相比,A1、A2、A3、A4、A5组PGDS和PGES蛋白表达及阳性表达率均升高(P<0.05),并随着瘢痕形成时间的增加而逐渐降低;A6组PGDS和PGES蛋白表达及阳性表达率与B组比较无统计学差异(P>0.05)。结论前列腺素可能是与增生性瘢痕形成有关的重要介质。Objective To investigate the expressions of prostaglandin D synthase （PGDS ） and prostaglandin E synthase （PGES ） in hyperplastic scar tissues .Methods According to different times of cicatrization ,47 patients were divided into six groups of A1 （5 h-2 d after burn ,9 cases） , A2（1 month after cicatrization ,9 cases） ,A3（3 months after cicatrization ,9 cases） ,A4（6 months after cicatrization ,8 cases ） ,A5 （12 months after cicatrization ,6 cases ） and A6 （24 months after cicatrization ,6 cases） .The normal skin tissue was taken as the control（group B ,10 cases） .The protein expressions of PGDS and PGES were detected by Western blot and immunohistochemical staining . Results Compared with group B ,the protein expressions and positive rates of PGDS and PGES were increasedin groups of A1,A2,A3,A4 and A5（P〈0.05）,which were gradually decreased with increasing times of cicatrization .Besides ,the protein expressions and positive rates of PGDS and PGES in group A6 were not statistically different from those in group B（P〉0 .05） .Conclusion Prosta-glandin may be an important mediator of hyperplastic scar .

关 键 词：增生性瘢痕 前列腺素D合成酶 前列腺素E合成酶 

分 类 号：R962[医药卫生—药理学]