果桑遗传差异的SRAP和EST-SSR分析  

SRAP and EST-SSR Analysis of Genetic Differences in Fruit Mulberry(Morus spp.)

作　　者：延娜[1] 李巧丽[1] 郭军战[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《西北农业学报》2015年第9期91-97,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省苗木繁育中心资助项目(14220304)

摘　　要：为了从分子水平揭示果桑种质资源间的亲缘关系，指导其种质资源的鉴定和利用，以及快速选育优良果桑新品种，利用sRAP和EST—SSR2种新型分子标记对供试果桑种质材料的遗传多样性进行分析。从42对SRAP引物中筛选出17对扩增条带清晰的引物，扩增得到306个位点，多态性位点达253个，多态性比率为82．68％，遗传相似系数（Gs）为0．3909～0．8111。从26对EST—SSR引物中筛选出18对引物，扩增出297个位点，多态性位点236个，多态性比率为79．46％，遗传相似系数（Gs）为0．5068～0．8581。综合2种分子标记得出供试材料的遗传相似系数（Gs）为0．4643～0．8143。利用uPGMA构建聚类树状图，最终供试材料被聚为3个类群，呈现出明显的地理分布特征，并由此得出SRAP分子标记更适用于果桑种质亲缘关系的遗传差异分析。In order to reveal the genetic relationship of fruit mulberry germplasm at molecular level and provide a scientific basis for germplasm identification, utilization and breeding novel quality varieties. SRAP and EST-SSR markers were used in this study to investigate the genetic diversity and relationship among fruit mulberry germplasm resources. The results showed that 306 bands were obtained by using 17 pairs of SRAP primers, which were selected from 42 pairs of SRAP primers , 253 bands were polymorphic, so the percentage of polymorphic bands was 82. 68% and the genetic similarity （GS） coefficient ranged from 0. 390 9 to 0. 811 1. Then 297 bands were also get amplification by using 18 pairs of selected EST-SSR primers from 26 pairs , of which 236 bands were polymorphic, so the percentage of polymorphic bands was 79.46% and the GS ranged from 0. 506 8 to 0. 858 1. Combining SRAP and EST-SSR markers, the GS of fruit mulberry germplasm resources ranged from 0. 464 3 to 0. 814 3. Based on the UPGMA-cluster analysis, all the materials were divided into three groups, which presented apparent characteristics of geographic distribution and SRAP markers were more effective in identifying genetic differentiation in fruit mulberry.

关 键 词：果桑 SRAP EST—SSR 遗传差异 

分 类 号：S663.9[农业科学—果树学]