抗凝血活性海洋硫酸多糖HP1-1的结构研究  

Structural characterization of the marine sulfated polysaccharide HP1-1 with anticoagulant activity

作　　者：李娜[1] 李菁[1] 刘雪[1] 王姝垚 曹素健 毛文君[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室山东省糖科学与糖工程重点实验室医药学院,山东青岛266003

出　　处：《中国海洋药物》2015年第5期7-12,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41476108);山东省科技发展计划项目(2014GHY115015);国家自然科学基金委员会-山东省人民政府联合资助海洋科学研究中心项目(U1406402)资助

摘　　要：目的对抗凝血活性海洋硫酸多糖HP1-1的结构进行研究。方法通过高效液相色谱(HPLC)、高效凝胶渗透色谱(HPGPC)、傅里叶变换红外光谱(IR)以及气质联用色谱(GC-MS)和核磁共振波谱(NMR)方法,对抗凝血活性海洋硫酸多糖HP1-1的结构进行解析。结果海洋硫酸多糖HP1-1的分子量为297kDa,总糖和硫酸基含量分别为78.1%和12.3%;HP1-1主要由葡萄糖组成,含有少量半乳糖、阿拉伯糖和甘露糖;HP1-1糖链中含有→4)-Glcp-(1→、→3)-Glcp-(1→、→3,4)-Glcp-(1→、→4)-Arap-(1→、→3,4)-Galp-(1→和→3)-Galp-(1→,硫酸基位于→4)-Glcp-(1→的C-3位和→3)-Galp-(1→的C-4位。结论海洋多糖HP1-1是主要由葡萄糖组成的硫酸多糖,含有多种糖基连接方式,为独特结构序列的新颖海洋硫酸甘露阿拉伯半乳葡聚糖。Objective To investigate the structural characterization of the marine sulfated polysaccharide HPI-1 with anticoagulant activity. Methods The structural characterization of the marine sulfated poly- saccharide HPI-1 with anticoagulant activity was investigated by high performance liquid chromatogra- phy （HPLC）, high gel permeation chromatography （HPGPC）, Fourier-transform infrared （IR）, gas chromatography-mass spectrometry （GC-MS） and NMR spectroscopy. Results The average molecular weight of the marine sulfated polysaccharide HPI-1 was estimated to be about 297 kDa. The contents of total sugar and sulfate ester of HPI-1 were 78. 1%and 12. 3 0%, respectively. The sulfated polysaccha- ride HPI-1 was mainly composed of glucose, with small amounts of arabinose, glactose and mannose. The chain of HPI-1 consisted of →4）-Glcp-（1→、→3）-Glcp-（1→、→3,4）-Glcp-（1→、→4）-Arap-（1→、→3,4）-Galp-（1→,→3）-Galp-（1→,and the positions of sulfate ester were at C-3 of-→4）-Glcp-（1→ and C-4 of →3）-Galp-（1→ Conclusion The marine sulfated polysaccharide HPI-1 was a glucose- containing sulfated polysaccharide with various linkage patterns, and could be a novel mannoarabinoga- lactoglucan distinguishing from other sulfated polysaccharides from marine algae.

关 键 词：硫酸多糖 化学组成 结构特征 

分 类 号：R917[医药卫生—药物分析学]