UHPLC-ESI-MS/MS法测定大鼠血浆中的山姜素和小豆蔻明  

Determination of alpinetin and cardamonin in rat plasma by UHPLC-ESIMS/MS

作　　者：陈伟康 熊蔚 许妍 方建和[2] 

机构地区：[1]江西省药品检验检测研究院,江西南昌330029 [2]江西中医药大学,江西南昌330004

出　　处：《中成药》2015年第10期2220-2224,共5页Chinese Traditional Patent Medicine

摘　　要：目的建立测定大鼠血浆中山姜素和小豆蔻明含有量的UHPLC-ESI-MS/MS法,并计算两者的药动学参数。方法血浆分析采用PhenomenexLuna C18色谱柱（150 mm×2.0 mm,3μm）;流动相为乙腈-0.1%甲酸水溶液（20∶80）;体积流量为0.4 m L/min;山姜素和小豆蔻明均采用正离子模式检测。结果标准品在1~1 000 ng/m L范围内线性关系良好,相关系数大于0.995,山姜素和小豆蔻明的最低检出限均为0.05 ng/m L,最低定量限均为0.2ng/m L,样品回收率在88.2%~99.3%之间。大鼠灌胃草豆蔻提取物后,两者的主要药动学参数Cmax分别为（385.633±91.192）和（186.683±69.893）ng/m L,t1/2z分别为（1.578±0.239）和（1.137±0.385）h,AUC（0-t）分别为（911.723±59.208）和（456.043±23.99）ng/m L/h,Vz/F分别为（24.295±6.858）和（35.606±12.887）L/kg。结论山姜素和小豆蔻明在大鼠体内的吸收和消除均比较迅速。AIM To establish a method for determining the contents of alpinetin and cardamonin in rat plasma by UHPLC-ESI-MS / MS and calculate their pharmacokinetic parameters. METHODS The analysis of plasma was performed on Phenomenex Luna C18column（ 150 mm × 2. 0 mm,3 μm） with acetonitrile-0. 1% formic acid aqueous solution（ 20 ∶ 80） as mobile phase,flow rate was 0. 4 m L / min,both alpinetin and cardamonin were detected in positive ion mode. RESULTS Calibration curves offered good linearities in the ranges of 1 ~ 1 000 ng / m L（ r 0. 995）. The detection limits of both alpinetin and cardamonin were 0. 05 ng / m L,while their quantitation limits were both 0. 2 ng / m L. The recoveries of samples were in the ranges of 88. 2% ~ 99. 3%. The main pharmacokinetic parameters of alpinetin and cardamonin in rats after intragastric administration were that Cmaxwere（ 385. 633 ± 91. 192） and（ 186. 683 ± 69. 893） ng /m L,t1 /2zwere（ 1. 578 ± 0. 239） and（ 1. 137 ± 0. 385） h,AUC（ 0- t）were（ 911. 723 ± 59. 208） and（ 456. 043 ± 23. 99） ng / m L / h, Vz/ F were（ 24. 295 ± 6. 858） and（ 35. 606 ± 12. 887） L/kg,respectively. CONCLUSION Both alpinetin and cardamonin are rapidly absorbed and eliminated in rats.

关 键 词：山姜素 小豆蔻明 大鼠血浆 药动学参数 UHPLC-ESI-MS/MS 

分 类 号：R284.1[医药卫生—中药学]