昌台牦牛κ-酪蛋白基因重复片段多态性的快速检测  

Rapid detection of κ-casein gene duplication fragment polymorphism in Changtai yak

作　　者：屈兵兵 金素钰[1] 蒋忠荣[2] 刘曦[2] 黄林[1] 郑玉才[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省甘孜州畜牧科学研究所,四川康定626000

出　　处：《西南民族大学学报（自然科学版）》2015年第5期529-532,共4页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：农业部行业专项(No.201203010)资助

摘　　要：分析昌台牦牛κ-酪蛋白基因重复片段多态性.用Chelex-100方法从昌台牦牛(n=100)全乳中提取基因组DNA,利用PCR对κ-酪蛋白基因第四外显子部分片段进行扩增,通过非变性PAGE及银染确定κ-酪蛋白基因重复片段多态性.结果显示昌台牦牛κ-酪蛋白基因重复片段存在A和B两种等位基因,基因频率分别为0.094,0.906,B等位基因包含12 bp的重复片段.AB型个体17个,BB型个体73个.昌台牦牛κ-CN基因重复片段等位基因B为优势等位基因.This study was to analyze Changtai yak kappa-casein gene duplication fragment polymorphism. Chelex-100 method was used to extract genomic DNA from whole milk of Changtai yaks （ u = 100）. Polymerase Chain Reaction was used to amplify the exon 4 region of K-casein gene, then the K-casein gene duplication fragment polymorphism was confirmed by non-denaturing polyaerylamide gel electrophoresis and silver-staining. The results showed that Changtai yak k-casein gene had A and B alleles of duplication fragment, with frequencies of 0.094 and 0.906, respectively, and B allele contained a 12-hp duplica- tion. Individuals carrying AB and BB genotypes were 17 and 73. B allele of K-casein gene is the dominant allele in Changtai yak.

关 键 词：昌台牦牛 κ-酪蛋白 遗传多态性 

分 类 号：S823.85[农业科学—畜牧学] S852.23[农业科学—畜牧兽医]