波棱瓜子对免疫性肝损伤的免疫炎症调节作用  

immune inflammation regulation of Herpetospermum seeds on immune liver injury

作　　者：刘思洋[1] 章雷[2] 顾健[1] 李佳川[1] 谭睿[1] 何黎黎[1] 张宏川[1] 孙宁阳[1] 

机构地区：[1]西南民族大学民族医药研究院,四川成都610041 [2]西南交通大学医学院,四川成都610031

出　　处：《西南民族大学学报（自然科学版）》2015年第5期555-560,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：国家自然科学基金面上项目(81674168;81573563)

摘　　要：研究建立Con A致小鼠免疫性肝损伤动物模型,采用免疫组化染色SP法考察不同剂量波棱瓜子总木脂素对小鼠肝脏组织CD4、CD8、CXCL10、ICAM-1、IRF-1、SOCS1、STAT1蛋白的表达,分析波棱瓜子总木脂素对抑制粘附分子和趋化因子肝内蛋白的表达影响,以期研究讨论藏药波棱瓜子总木脂素对免疫性肝损伤的免疫炎症调节作用.实验结果表明,波棱瓜子总木脂素对Con A致免疫性肝损伤小鼠的CD4、ICAM-1、IRF-1蛋白含量无明显差异;而对CD8、CXCL10、SOCS1和STAT1蛋白的含量有不同程度的降低,显示出通过调节T细胞的免疫功能来保护肝脏.由上述结果可知,波棱瓜子总木脂素具有一定的免疫性炎症调节作用,该作用可能是波棱瓜子总木脂素对肝脏免疫性疾病的部分保护机制.This paper established a immune liver injury model in mice induced by ConA, and investigated the expression of CIM, CDS, CXCL10, ICAM - 1, IRF - 1, SOCS1, STAT1 proteins in liver tissue under different doses of Herpetospermum seeds total lignans by immunhistochemistry SP method, and discussed the regulation effect of Tibetan seeds Herpetospermum total lig- nans on immune and inflammatory in immune injury liver. The results showed that the influence of Herpetospermum seeds total lignans on the content of protein CIM, ICAM - 1, IRF - 1 in immunological liver injury mice hadno significant difference ; While CDS, CXCL10, SOCS1 and STAT1 protein contents exhibited different degrees of reduction, showing that it protects liver via the regulatory of T cells. All these results indicate that Herpetospermum seeds total lignans have some regulation on immune inflam- mation,which may be a part of protection mechanisms on immune liver injury.

关 键 词：波棱瓜子 总木脂素 免疫性肝损伤 免疫炎症调节作用 

分 类 号：R285[医药卫生—中药学] R29[医药卫生—中医学]