OPN调节IL-17/NF-κB通路参与疲劳性骨损伤的初步研究  

Roles of OPN in bone fatigue damage by regulating IL-17/NF-κB pathway

作　　者：王增田[1] 邢杰[2] 兰晓霞[3] 张秋燕[1] 王翼腾 曹振兰 李覃[2] 

机构地区：[1]天津武警后勤学院临床医学系,300309 [2]天津武警后勤学院病原生物与免疫学教研室,300309 [3]天津武警后勤学院救援医学系,300309

出　　处：《免疫学杂志》2015年第10期868-872,共5页Immunological Journal

基　　金：武警后勤学院科研基金(WHJ201405);国家自然科学基金(81202843);天津市应用基础与前沿技术研究计划重点项目(14JCZDJC36700);天津市心血管重塑与靶器官损伤重点实验室开放基金(TJC1402)

摘　　要：目的初步探讨骨桥蛋白(osteopontin,OPN)在疲劳性骨损伤中的作用。方法雌性SD大鼠随机分为正常对照组和跑台训练组(n=10),6周后ECT检查大鼠骨核素显像的变化,ELISA检测血清IL-17含量,RT-PCR及Western blot检测OPN、NF-κB/p65的表达水平。结果全部训练结束时,大鼠处于疲劳状态,与正常对照组相比,训练组大鼠胫骨出现核素积聚,血清IL-17含量明显增加,同时,OPN的基因和蛋白表达及NF-κB/p65表达水平均显著升高。结论 OPN参与疲劳性骨损伤的病程进展,可能与IL-17及NF-κB通路密切相关,为发现疾病新的诊断标志物及治疗靶点提供研究思路。This study aimed to explore the roles of osteopontin（OPN） playing in the development of bone fatigue damage. Female Sprague-Dawley（SD） rats were randomly divided into normal control group and treadmill training group（n =10）. Six weeks later, image analysis of ECT was carried out to observe the changes of bone.Meanwhile, the level of IL-17 in serum and the expression levels of OPN and NF-κB/p65 were detected by ELISA,RT-PCR and Western blot. Compared with the normal group, the rats of treadmill training group were tired, and presented higher concentration of radionuclide in the tibia at the end of the experiment. Furthermore, the levels of IL-17, OPN and NF-κB/p65 in treadmill training group were much higher than those in normal group. Taken together, the development of bone fatigue damage might be closely related to OPN and IL-17/NF-κB pathway, and this finding may provide a novel strategy for the seeking of diagnostic marker and therapeutic target in bone fatigue damage.

关 键 词：跑台训练 骨桥蛋白 IL-17 炎症 NF-ΚB 

分 类 号：R873[医药卫生—运动医学]