新疆部分地区麻疹野病毒分离与基因特征分析  

Analysis on isolation and genotyping of wild measles virus in some areas of Xinjiang

作　　者：关静[1] 任丽[2] 陈涛[1] 郭强[1] 王慧玲[2] 甫尔哈提[1] 关晓冬[1] 张燕[2] 

机构地区：[1]新疆维吾尔自治区疾病预防控制中心,新疆乌鲁木齐830011 [2]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《疾病监测》2015年第9期723-726,共4页Disease Surveillance

基　　金：新疆维吾尔自治区卫生厅青年科技人才专项科研项目(No.2013Y05)~~

摘　　要：目的探讨新疆目前流行的麻疹病毒的基因型别和特征。方法采用Vero/SLAM细胞分离培养麻疹病毒,通过反转录-聚合酶链反应方法扩增麻疹病毒N基因羧基末端456 bp片段,并对PCR产物进行序列测定和同源性分析。结果 2013 2014年新疆分离到7株麻疹病毒均为H1a基因亚型,与Shanghai-191同源性为89.5%~87.3%;与世界卫生组织推荐用于鉴定基因型别的6株H1基因型同源性为91.0%~99.3%,与目前3株流行H1a亚型同源性为97.3%~100.0%。结论 H1a基因亚型仍为新疆本土流行病毒株的优势亚型,近年来引起新疆麻疹流行的麻疹病毒基因序列存在着不同程度的差异,可能来自于不同的传播链,麻疹病毒株H1a亚型的持续传播与易感人群的累积和人口流动有密切关系。Objective To explore the genetic characteristics and genotype of measles virus circulating recently in Xinjiang. Methods Vero/SLAM cell were used for measles viral isolation and culture. A 456 bp fragment of the N gene was amplified with reverse transcription polymerase chain reaction（ RT-PCR） and the sequence identification and homological analysis were conducted. Results Seven strains of measles virus were isolated in Xinjiang during 2013-2014 and all the strains were identified as subtype H1 a. The strains shared 87. 3%- 89. 5% homology of nucleotides sequence with Shanghai-191 vaccine strain,shared 91. 0%- 99. 3% homology of nucleotides sequence with six genotype H1 strains recommended for genotype identification by WHO,and shared 97. 3%- 100. 0% homology of nucleotides sequence with 3 circulating strains of subtype H1 a strains. Conclusion Measles virus subtype H1 a was predominant in Xinjiang. Recent measles epidemics were caused by the viruses with different gene sequences in Xinjiang. The continuous spread of subtype H1 a strain is closely related to the accumulation of susceptible populations and population mobility.

关 键 词：麻疹病毒 基因型 序列分析 

分 类 号：R373.11[医药卫生—病原生物学]