PI3P在暗诱导的蚕豆气孔关闭中的作用及与H_2O_2和NO的关系  

Role of PI3P and Its Relationship with H_2O_2 or Nitric Oxide in Darkness-induced Stomatal Closure in Vicia faba

作　　者：马敏[1] 刘艾京 胡洁[1] 贺军民[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《植物学报》2015年第5期583-590,共8页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31170370);中央高校基本科研业务费(No.GK201401005)

摘　　要：以蚕豆(Vicia faba)表皮条为材料,利用磷脂酰肌醇3-激酶(PI3K)的抑制剂沃曼青霉素(WM)和LY294002(LY)抑制磷脂酰肌醇3-磷酸(PI3P)的形成,并结合气孔开度分析及激光扫描共聚焦显微镜技术,探讨暗诱导蚕豆气孔关闭过程中PI3P与过氧化氢(H2O2)和一氧化氮(NO)之间的相互关系。结果表明,WM和LY显著抑制暗诱导的保卫细胞H2O2和NO的形成以及气孔的关闭,但不能抑制外源H2O2和NO诱导的气孔关闭,外源H2O2和NO处理能完全逆转WM和LY对暗诱导的气孔关闭的抑制效应。实验结果暗示,在暗诱导的气孔关闭的信号转导途径中PI3P在信号分子H2O2和NO的上游起作用。In combination with physiological and cell biological research approaches including stomatal bioassay and laser-scanning confocal microscopy, we studied the interrelationship of phosphatidylinositol 3-phosphate（PI3P） and H2O2 or nitric oxide（NO） in darkness-induced stomatal closure in epidermal strips of Vicia faba（broad bean） by using the drug treatment with wortmannin（WM） and LY294002（LY） to inhibit the production of PI3 P. The obtained results showed that both WM and LY significantly inhibited darkness-induced synthesis of H2O2 and NO in guard cells and subsequent stomatal closure but not exogenous H2O2- and NO-induced stomatal closure. In addition, exogenous treatment with H2O2 and NO completely reversed the inhibitory effect of WM and LY on darkness-induced stomatal closure. In conclusion, our results suggest that PI3 P may function upstream of H2O2 and NO in the signal transduction pathways of darkness-induced stomatal closure in V. faba.

关 键 词：黑暗 过氧化氢 一氧化氮 磷脂酰肌醇3-磷酸 气孔关闭 蚕豆 

分 类 号：S643.6[农业科学—蔬菜学]