以黄花乌头发根为外植体的再生培养体系建立  

Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum

作　　者：刘莉莉[1] 卢淑波[1] 徐佳萍[1] 张庆田[1] 李昌禹[1] 

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《植物学报》2015年第5期623-627,共5页Chinese Bulletin of Botany

基　　金：吉林省科学技术厅自然科学基金(No.201115131)

摘　　要：为获得黄花乌头(Aconitum coreanum)发根再生植株,将其发根在添加0.5–5.0 mg·L–1 6-BA及0–0.5 mg·L–1 NAA的1/2MS固体培养基上进行光诱导培养,发根经脱分化形成愈伤组织,然后形成芽及芽丛,最终获得发根再生植株。结果表明,愈伤组织及不定芽分化的最适培养基为1/2MS+2.0 mg·L–1 6-BA+0.2 mg·L–1 NAA+3%蔗糖;不定芽生根最适培养基为1/2MS+0.5 mg·L–1 6-BA+0.1 mg·L–1 NAA+5 mg·L–1 GA+3%蔗糖。The hairy roots of Aconitum coreanum were used as explants to induce callus via differentiation in 1/2MS medium with 0.5–5 mg·L^–1 6-BA and 0–0.5 mg·L^–1 NAA, then regeneration plantlets were induced from the callus. A whole transgenetic plantlet was obtained. The optimal medium for inducing callus and adventitious buds was 1/2MS＋2.0 mg·L^–1 6-BA＋0.2 mg·L^–1 NAA＋3% sugar. The optimal medium for inducing roots was 1/2MS＋0.5 mg·L^–1 6-BA＋0.1 mg·L^–1 NAA＋5 mg·L^–1 GA＋3% sugar.

关 键 词：黄花乌头 发根 植株再生 培养体系 

分 类 号：S567.239[农业科学—中草药栽培]