微生态制剂对刺参幼参养殖水质指标和生长、相关酶活性的影响  

Effect of Microecologics on the Growth and Enzyme Activity of Juvenile Sea Cucumber(Apostichopus japonicus)and Water Quality

作　　者：王圣[1,2] 王忠全 李斌 刘相全 相智巍 宋向军 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]山东省海洋资源与环境研究院山东省海洋生态修复重点实验室,山东烟台264006

出　　处：《海洋湖沼通报》2015年第3期71-77,共7页Transactions of Oceanology and Limnology

基　　金：海洋公益性行业科研专项经费项目(201305001;201305005);山东省科技发展计划项目(2013GHY11532);山东省海洋经济创新发展区域示范项目(20130125);水生动物营养于饲料"泰山学者"岗位经费资助

摘　　要：以刺参幼参为试验对象,在其基础饲料中分别添加0(对照)、0.2、1和5g/kg的微生态制剂作为免疫增强剂,进行28天的养殖实验,研究其对育苗水体中氨氮和亚硝态氮浓度及刺参生长、消化和免疫酶活性的影响。结果表明:28d时,微生态制剂对于刺参养殖水体氨氮和亚硝态氮具有明显的去除效果(P<0.05);对刺参生长具有一定促进作用,其中1g/kg组幼参特定生长率最高,但与其他组差异不显著(P>0.05);处理组的消化酶(淀粉酶、蛋白酶)与对照组相比均有显著提高(P<0.05),免疫酶(碱性磷酸酶、超氧化物歧化酶)与对照组相比也有显著的提高(P<0.05)。该免疫增强剂添加量为5g/kg时,对降低育苗水体氨氮浓度和亚硝浓度及增强刺参幼参消化和免疫活力的效果最佳。本研究的结果可为微生态制剂在刺参育苗与养殖中的应用提供参考。By a 28-day feeding trial of juvenile sea cucumber （Apostichopus japonicus）, the effects of microecologics （basic feed supplemented with 0, 0.2, 1, 5 g/kg of microecologics,respectively） on am- monium and nitrate concentrations of breeding seawater and as the immunostimulant on the growth and enzyme activity of A. japonicus were investigated. The results showed that this microecologics clearly decreased the ammonium and nitrate nitrogen concentration seawater （P〈0.05） on day 28. Although it did not significantly promote the growth and survival rate （P〈0.05）, it apparently enhanced AKP, SOD, AMS and pepsin activities of treament groups （P〈0.05）. In brief, group 5 g/kg obtained the best result almost on all parameters. The result of this study are expected to provide a reference for the application of microecologics in Apostichopus japonicus breeding and aquaculture.

关 键 词：刺参 免疫增强剂 无机氮 生长 酶活性 

分 类 号：S968.9[农业科学—水产养殖]