基于SNP标记的玉米容重QTL分析  

QTL Identification for Test Weight Based on SNP Mapping in Maize

作　　者：许理文[1,2] 段民孝[2] 田红丽[2] 宋伟[2] 王凤格[2] 赵久然[2] 刘保林[2] 王守才[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室,北京100097

出　　处：《玉米科学》2015年第5期21-25,共5页Journal of Maize Sciences

基　　金：国家科技支撑项目(2011BAD35B09;2014BAD01B09);北京市农林科学院科技创新能力建设专项(KJCX20140202);"863"计划"玉米产量功能基因研究与克隆"(2012AA10A305)

摘　　要：以240个DH系为作图群体,利用SNP芯片对DH群体进行基因型分析,构建连锁图谱,对DH群体进行容重性状鉴定。结果表明,采用复合区间作图法进行QTL定位分析,在4个环境下共检测到5个控制玉米容重的QTL,联合贡献率为23.61%;位于第1染色体的q Tw1-1介于SNP标记PZE-101032246与SYN13192之间,位于第9染色体的q Tw9-1介于SNP标记PZE-109011840和SYN6085之间,分别解释11.9%和7.8%的表型变异,表明这些区域可能包含调控玉米容重性状关键基因。A mapping population consisting of 240 DH lineswas constructed, and significant genotypic varia-tion for maize test weight was observed in 4 environments. Based on the genetic map containing, QTL for maize testweight were detected by composite interval mapping. Five QTLs were identified, and together explained 23.61% ofthe phenotypic variation. q Tw1-1 was detected on chromosome 1, locating in SNP of interval PZE- 101032246-SYN13192, q Tw9-1 was detected in SNP of interval PZE-109011840-SYN6085 on chromosome 9, with explained11.9% and 7.8% of the phenotypic variation respectively. The results showed that some key genes for test weightwere possibly contained in these regions.

关 键 词：玉米 容重 数量性状位点 单核苷酸多态性 加倍单倍体 

分 类 号：S513.035.3[农业科学—作物学]