玉米opaque2近等基因系中相关基因的表达量分析  

Transcription Analysis of Gene Expression in Near Isogenic Lines of opaque2 in Maize

作　　者：颜娜[1] 宋丽雅[2] 夏仁培 周志强[1] 张世煌[1] 李新海[1] 郝转芳[1] 翁建峰[1] 张德贵[1] 雍洪军[1] 李明顺[1] 

机构地区：[1]中国农业科学院作物科学研究所,农业部遗传育种重点实验室,北京100081 [2]北京工商大学/北京市植物资源研究开发重点实验室,北京100048

出　　处：《玉米科学》2015年第5期26-32,共7页Journal of Maize Sciences

基　　金：国家自然基金(31401390);国家现代农业产业技术体系建设专项(CARS-02-01)

摘　　要：Opaque2(O2)基因编码产生OPAQUE2蛋白,该蛋白为碱性亮氨酸拉链家族的一种转录因子,可作用于基因的启动区,调控基因的转录。辽2345/o2为本实验室构建的辽2345的opaque2(o2)基因回交导入系,蛋白组学分析表明,辽2345与辽2345/o2在授粉后18 d的胚乳细胞中表达蛋白的种类和数量存在很大差异,从中筛选出差异较明显的9种蛋白质。为了进一步验证O2与9种蛋白间的作用关系,应用相对荧光定量PCR的方法对9种蛋白进行了RNA水平表达量的检测。结果表明,在胚乳发育过程中Chfi很可能受到O2的抑制作用,O2很可能对Ubc、Lgl、Sdh、LOC541920、Shmt、Ppdk等基因存在某种促进或激活作用。OPAQUE2, encoding by gene Opaque2, is one member of alkaline leucine zipper transcription factorfamily, and can regulate the expressions of several related genes by combining with the promoter region. Liao2345/o2 is the gene backcross import department of o2 constructed by the laboratory. Obvious differences between protein ex-pressions were verified by analyzing protein expressions in the endosperm of 18 days after pollination（DAP） usingthe method of two-dimensional electrophoresis, and 9 proteins were screened out through the mass spectrometryanalysis. In order to determine the relationship between Opaque2 and the selected proteins, the amount of the RNAlevel expression of the selected proteins wasdetected using the relative fluorescence quantitative PCR（q RT-PCR）.The result showed that Chif may be suppressed by O2, whereas Ubc, Lgl, Sdh, LOC541920, Shmt and Ppdk werelikely promoted or activated by O2.

关 键 词：玉米 近等基因系 Opaque2 相对表达量 

分 类 号：S513.035.3[农业科学—作物学]