针刺对自发性高血压大鼠血压和抗氧化酶SOD、GSH-PX的影响  

The effects of acupuncturing on spontaneously hypertensive rats blood pressure and antioxidant enzymes SOD&GSH-PX

作　　者：刘健[1,2,3,4] 万基伟 高婷[1,5] 李银虹[1,5] 

机构地区：[1]天津中医药大学第一附属医院针灸研究所,天津300193 [2]国家中医药管理局脑病针刺疗法重点研究室 [3]国家中医药管理局针刺量效关系三级实验室 [4]天津市针灸学重点实验室,天津300193 [5]天津中医药大学,天津300193

出　　处：《陕西中医》2015年第10期1429-1431,共3页Shaanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81202738);教育部"长江学者和创新团队发展计划"创新团队资助;天津市应用基础与前沿技术研究计划(13JCZDJC31700);天津市卫生局中医中西医结合科研项目(11036)

摘　　要：目的：观察针刺对自发性高血压大鼠（SHR）血压的影响,从抗氧化酶角度探讨针刺降压机制。方法：将SHR随机分为针刺组、模型组,以京都Wistar大鼠（WKY）作为血压正常的对照组。针刺组取双侧人迎穴,施以捻转手法,捻转频率为120~160次/min,行针1min,每日针刺1次;模型组和正常组进行与针刺组相同时间、相同程度的捉抓。每天针刺前后测量收缩压和舒张压。针刺20d后,取胸主动脉,用黄嘌呤氧化酶比色法测定SOD活性,化学比色法测定GSH-PX活性。结果：针刺20d后SHR收缩压和舒张压均较针刺前降低,且均低于模型组。针刺组SOD和GSH-PX活性较模型组均有升高的趋势。结论：针刺可降低SHR血压,其机制可能与提高SOD和GSH-PX活性,从而提高机体抗氧化能力有关。Objective：To observe the effects of acupuncture on SHR blood pressure,and research the mechanisms from the perspective of antioxidant enzymes.Method：To separate the SHRs into acupuncture group and model group randomly,WKYs as normal blood pressure controlled group.To acupuncture group,acupuncture with the twirling manipulation,frequency120-160/min,lasting 1min.Model group and normal group were treated by the same crawl stimulation.The systolic pressure was measured before and after the treatment every day.After 20 days of treatment,all the rats were decapitated and got the thoracic aorta.The activity of SOD and GSH-7PX was measured by xanthine oxidase colorimetric method and chemical colorimetry.Results：After 20 days acupuncture,in acupuncture group,the both systolic and diastolic blood pressure of SHR were lower than before,and lower than model.The activity of SOD and GSH-PX in acupuncture were rising trend compared with model group.Conclusion：Acupuncture can reduce SHR systolic and diastolic blood pressure and its mechanisms may be related to improve the activity of SOD and GSH-PX,thus enhance the antioxidant capacity.

关 键 词：高血压/针刺疗法 动物 实验 大鼠 

分 类 号：R544.1[医药卫生—心血管疾病]