白芍水提物的抗炎作用及作用机制研究  

The Anti-inflammatory Effect and Mechanisms of the Water Extract of White Peony Root

作　　者：王双[1] 臧志和[1] 彭延娟[1] 

机构地区：[1]成都医学院药学院,成都610083

出　　处：《四川动物》2015年第5期748-751,共4页Sichuan Journal of Zoology

基　　金：四川省教育厅高等教育质量工程子项目(201101102)

摘　　要：目的与白芍总苷进行比较,研究白芍水提物的抗炎作用,探讨其抗炎作用的机制。方法采用二甲苯致小鼠耳片肿胀模型和蛋清致大鼠足跖肿胀模型,观察白芍水提物和白芍总苷对急性炎症的抗炎作用。采用琼脂致小鼠肉芽肿模型,观察白芍水提物和白芍总苷对慢性炎症的抗炎作用;通过测定琼脂致小鼠肉芽肿模型血清前列腺素E2(PGE2)水平,蛋清致大鼠足跖肿胀模型肿胀足的一氧化氮(NO)、丙二醛(MDA)及PGE2含量,探究其抗炎作用的部分机制。结果白芍水提物4 g·kg-1、白芍总苷180 mg·kg-1均可显著抑制二甲苯致小鼠耳片肿胀和琼脂肉芽肿(P<0.05),降低琼脂致小鼠肉芽肿模型血清PGE2水平(P<0.05)。白芍水提物2.8 g·kg-1、白芍总苷120 mg·kg-1均可显著抑制蛋清致大鼠足跖肿胀(P<0.05),降低肿胀足的NO、MDA及PGE2含量(P<0.05);白芍水提物和白芍总苷对急、慢性炎症的抑制作用差异无统计学意义(P>0.05)。结论白芍水提物具有一定的抗炎作用,其抗炎机制可能与减少血液和局部组织PGE2水平,减少局部组织NO、MDA含量有关。Objective To study the anti-inflammatory effect and mechanisms of the water extract of white peony root in comparison with the total glucosides of paeonia（ TGP）. Methods The anti-inflammatory effects of the water extract of white peony root and TGP were measured in three models： mice ear swelling induced by xylene,rats paw swelling induced by albumen and mice chronic granuloma induced by agar. Subsequently,the levels of PGE2 in the serum of agar-induced granuloma mice,and the levels of NO,MDA and PGE2 in swelling paw of albumen-induced rats were measured. Results The water extract of white peony root（ 4 g·kg- 1） and TGP（ 180 mg·kg- 1） could significantly inhibit mice ear swelling and agar-induced chronic granuloma,and reduce the level of PGE2 in mice serum. The water extract of white peony root（ 2. 8 g·kg- 1） and TGP（ 120 mg·kg- 1） could significantly inhibit rats paw swelling,and reduce the rats levels of NO,MDA and PGE2 in the rats paw swelling model. Conclusion The water extract of white peony root has anti-inflammatory effects,which may be due to the inhibition on the production of NO,PGE2 and MDA.

关 键 词：白芍水提物 白芍总苷 抗炎机制 一氧化氮 丙二醛 前列腺素E2 

分 类 号：R285.5[医药卫生—中药学]