藏药复方“吉尼德协”的质量标准研究  

Study on Quality Standard of Tibetan Medicine Ji-Ni De-Xie

作　　者：李艳[1] 雄呷 吕秀梅[1] 伍金丹增 段炼[1] 范刚[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137 [2]德格县藏医院藏医药研究所,德格627250

出　　处：《世界科学技术-中医药现代化》2015年第7期1551-1555,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委青年基金项目(81303310):基于DNA条形码和1H-NMR代谢组学技术的多基源藏药小檗皮二维鉴定体系的构建;负责人:范刚;四川省中医药管理局一般项目(2013-03):藏药院内制剂的二次开发研究;负责人:张艺;范刚

摘　　要：目的:建立藏药复方"吉尼德协"的薄层鉴别及含量测定方法。方法:采用薄层色谱(TLC)法对方中的小檗皮、诃子、余甘子和姜黄进行鉴别,采用高效液相(HPLC)法测定方中盐酸小檗碱的含量。结果:TLC鉴别特征明显,斑点清晰,且阴性无干扰;在0.05-0.45 mg·m L-1范围内,盐酸小檗碱与峰面积具有良好的线性关系。回归方程为Y=40 679X–130.56,平均加样回收率为99.07%。结论:所建立的方法操作简单,重复性良好,能有效控制藏药复方"吉尼德协"的质量。This study was aimed to establish the thin layer chromatography （TLC） identification and content determination of Tibetan medicineJi-Ni De-Xie （JNDX）. TLC was used to identifyBerberis kansuensisSchneid,Terminalia chebula Retz.,Phyllanthus emblicaandCurcuma LongaL. The high performance liquid chromatography （HPLC） was used to determine the content of berberine hydrochloride. The results showed that the TLC identification had a good resolution with clear spots and no interference from the negative reference. The content of berberine hydrochloride showed a good linear relationship with the peak area in the range of 0.05-0.45 mg＆#183;mL-1. The regression equation wasY = 40 679X-130.56. The average recovery was 99.07%. It was concluded that the established method was easy to operate with good repeatability. It can effectively control the quality of Tibetan medicine JNDX.

关 键 词：藏药 吉尼德协 薄层色谱法 高效液相色谱法 盐酸小檗碱 

分 类 号：R284.1[医药卫生—中药学]