利用高密度SNP标记定位水稻粒形相关QTL  

Mapping of Rice Grain Shape Relevant QTLs Using High-density SNP Markers

作　　者：胡苗[1,2] 孙志忠[2] 孙学武[2] 谭炎宁[2] 余东[2] 刘瑞芬[2] 袁贵龙[2] 丁佳[2] 袁定阳[1,2] 段美娟[1,2,3] 

机构地区：[1]中南大学研究生院隆平分院,湖南长沙410125 [2]湖南杂交水稻研究中心,湖南长沙410125 [3]湖南省农业科学院,湖南长沙410125

出　　处：《杂交水稻》2015年第5期54-58,共5页Hybrid Rice

基　　金：湖南省自然科学基金杰出青年项目(2015JJ1026);国家"863"计划(2014AA10A604);长沙市科技局项目(K1403015-21)

摘　　要：以"巨穗稻"R1128与粳稻品种日本晴杂交,构建包含781个单株的F2分离群体,利用高通量测序技术开发高密度SNP标记并构建超高密度的遗传连锁图谱,对水稻粒长、粒宽和粒厚3个性状进行QTL定位分析。共检测到分布于除第1,11,12号染色体以外的9条染色体上的19个粒形相关QTL,其中10个控制粒长,5个控制粒宽,4个控制粒厚,这些QTL中,粒长QTL q GL4-2、q GL7-2、q GL10-2、q GL10-3,粒宽QTL q GW6,粒厚QTL q GT4、q GT8可能为新发现的粒形相关位点。An F2 segregation population containing 781 single plants derived from the cross between Jushuidao R1128 with giant panicles and Nipponbare,a japonica rice variety,was established to develop high-density SNP markers by the high-throughput sequencing technique and construct ultrahigh-density genetic linkage map for the QTL mapping analysis of three rice grain shape characters including grain length,width and thickness. A total of19 grain shape relevant QTLs were detected on nine chromosomes except Chromosomes 1,11 and 12. Ten of them were responsible for controlling grain length,five for controlling grain width and four for controlling grain thickness.Among these QTLs,grain length QTLs q GL4-2,q GL7-2,q GL10-2 and q GL10-3,grain width QTL q GW6 and grain thickness QTLs q GT4 and q GT8 were likely to be newly discovered grain shape relevant loci.

关 键 词：水稻 粒形 高通量测序 SNP标记 QTL定位 

分 类 号：Q75[生物学—分子生物学] S511[农业科学—作物学]