多囊卵巢综合征大鼠卵巢HSP70及HSP90的表达研究  

Expression of Heat Shock Protein 70 and 90 in Ovary Tissues of Rats with Polycyst Ovarian Syndrome

作　　者：吴庚香[1] 尹太郎[1] 杨菁[1] 徐望明[1] 李维[1] 余楠[1] 邹宇洁[1] 张博[1] 王雅琴[1] 

机构地区：[1]武汉大学人民医院生殖医学中心,武汉市430060

出　　处：《医学分子生物学杂志》2015年第5期263-266,共4页Journal of Medical Molecular Biology

基　　金：中央高校青年教师专项经费项目（No.2042015kf0125）

摘　　要：目的利用免疫组化法检测多囊卵巢综合征（PCOS）大鼠卵巢中热休克蛋白（heatshockpro．rein，HSP）70及HsPg0的表达情况。方法将23日龄雌性SD大鼠分为PCOS组及对照组，PCOS组以硫酸普拉睾酮钠90μg/（g·d）皮下注射20d，同时给予高脂高糖饲料喂养；正常对照组大鼠每天皮下注射等量注射用水。DHEA注射完12h后，处死大鼠，观察卵巢形态学改变，测定血清性激素，免疫组化染色检测各组大鼠卵巢组织中HSP70及HSP90的表达。结果PCOS组大鼠雌激素、雄激素及卵泡刺激素水平均明显高于正常对照组。免疫组化法发现正常大鼠卵巢组织中均有HSP70及HSP90的表达．主要为胞质表达．PCOS组大鼠卵巢中HSP70的表达较正常组明显增强，而HSP90的表达则低于正常组。结论大鼠卵巢中存在HSPT0及HSP90的表达，且在PCOS大鼠卵巢中两种蛋白的表达存在差异。HSP70及HSP90的差异表达可能在PCOS的病理发展中具有一定的作用。Objective To examine the expression of heat shock protein （HSP） 70 and 90 in o- vary tissues of rats with polycyst ovarian syndrome （PCOS） by immunohistochemistry （IHC） ~ Methods Female SD rats aged 23 days were divided into two groups： control group and PCOS group. The rats in PCOS group were injected subcutaneously with sodium prasterone sulfate at 90 μg/ （g · d） daily for 20 days, while those in the control group were given equal water for injec- tion. All rats were fed on high-fat and high-sugar diet. They were sacrificed 12 hours after DHEA in- jection. The pathological changes of ovarian tissues were observed. The serum levels of hormones were measured. IHC staining for HSP70 and HSP90 was performed in ovary tissues. Results The levels of estrogen, androgen and follicle-stimulating hormone were significantly increased in PCOS group than in control group （ P 〈 0. 05 ） . IHC staining showed that HSP70 and HSP90 were ex- pressed in the cytoplasm of cells in ovary tissues of normal rats. The expression level of HSP70 was significantly increased and that of HSP90 deceased in PCOS group when compared with those in nor- mal group （P 〈 0. 05） . Conclusion HSP70 and HSP90 are expressed in ovary tissues of normal rats. Their expressions in ovary tissues of PCOS rats are greatly different from those in normal rats, indication that the expressions of HSP70 and HSP90 are involved in the development of PCOS.

关 键 词：多囊卵巢综合征 热休克蛋白70 热休克蛋白90 

分 类 号：R711.51[医药卫生—妇产科学]