黄芪提取物对大鼠骨髓源性内皮祖细胞的作用  

Effect of astragalus extracts on bone marrow-derived endothelial progenitor cells in rats

作　　者：刘暖[1] 毛秉豫[1] 杨雷[1] 徐国昌[1] 叶松山[1] 张培华[1] 张瓅芳 

机构地区：[1]南阳理工学院医学实验中心,473004

出　　处：《天津医药》2015年第10期1093-1096,I0001,共5页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81473438;81202791;81173372);河南省中医内科学重点学科支撑课题(豫教高[2012]186号)

摘　　要：目的探讨黄芪提取物对大鼠骨髓源性内皮祖细胞(EPCs)黏附、迁移、活力、血管形成能力及内皮型一氧化氮合成酶(e NOS)表达的影响。方法体外培养、分离和鉴定EPCs,设10-4、10-3、10-2g/L黄芪提取物组和对照组。倒置显微镜下观察并比较各组EPCs黏附、迁移、血管形成能力的差异;MTT法检测EPCs的活力变化;RT-PCR法检测EPCs中e NOS m RNA的表达;Western blot检测EPCs中e NOS蛋白的表达。结果与对照组相比,不同浓度黄芪提取物组促EPCs黏附、迁移、血管形成能力均显著增强,且呈浓度依赖性(F值分别为15.256、13.633、97.549,均P<0.05);EPCs的活力显著增加,呈时间(F时间=9.755)和浓度依赖性(F组间=10.018);且EPCs中e NOS m RNA和蛋白的表达水平均明显升高,呈浓度依赖性(F值分别为56.356、77.125,均P<0.05)。结论黄芪提取物具有调控EPCs促血管新生的作用,该作用可能与上调e NOS的表达水平密切相关。Objective To explore the effect of astragalus extracts on adherence, migration, cell viability, microvascular formation, and eNOS expression in bone marrow-derived endothelial progenitor cells （EPCs） of rats. Methods EPCs were cultured, isolated and identified in vitro and divided into four groups： low titer group （10^-4 g/L of astragalus extracts）, middle titer group （10^-3 g/L of astragalus extracts）, high titer group （10^-2 g/L of astragalus extracts） and control group. The effects of astragalus extract on adhesion, migration or microvascular formation were observed under the inverted microscope and com-pared between all groups;Cell viability was detected by MTT assay;mRNA transcription and protein expression levels of en-dothelial nitric oxide synthase （eNOS） in EPCs were detected by reverse transcription PCR （RT-PCR） and Western blot re-spectively. Results Compared with the control group, cell adhesion, migration and microvascular formation of EPCs all en-hanced with addition of astragalus extracts in a dose dependent manner （F=15.256, 13.633, 97.549 respectively, and P 〈0.05 in all cases）; Cell vitality of EPCs increased with administration of astragalus extracts in a time and dose dependant manner. （F=9.755 for time and F=10.18 for dose）. mRNA transcription and protein expression of eNOS in EPCs were up-reg-ulated with addition of astragalus extracts in a dose dependent manner （F=56.356 and 77.125 respectively, and P〈0.05 in both cases）. Conclusion Astragalus extracts play important role in angiogenesis in EPCs probably through up-regulating expressions of eNOS.

关 键 词：黄芪 骨髓祖代细胞 细胞黏附 细胞运动 内皮祖细胞 血管新生 内皮型一氧化氮合成酶 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R322.12[医药卫生—基础医学]