荷丹片对APOE^(-/-)小鼠炎症因子及氧化应激因子的影响  

Effects of Hedan tablet on cytokins and oxidative stress factors in APOE^(-/-) mouse

作　　者：于柏青[1] 周玉娟 刘福林[3] 曹晓华[3] 许明轩 

机构地区：[1]河北大学研究生院,河北保定071000 [2]基础医学院药理教研室 [3]河北大学附属医院 [4]沧州市人民医院

出　　处：《天津医药》2015年第10期1144-1146,共3页Tianjin Medical Journal

基　　金：河北大学医学学科专项基金建设项目(2012A3001)

摘　　要：目的探讨荷丹片对载脂蛋白E基因敲除(APOE-/-)小鼠炎症因子、氧化应激因子的影响及对动脉粥样硬化(AS)的干预作用及机制。方法将50只APOE-/-小鼠随机均分为对照组、模型组、荷丹片低剂量组、荷丹片高剂量组及辛伐他汀组。对照组给予普通饲料,其余组给予高胆固醇饲料造模。同时用药组灌胃相应剂量药物,对照组和模型组给予等量生理盐水灌胃。造模12周后测定血清白细胞介素(IL)-1、IL-10、丙二醛(MDA)、超氧化物歧化酶(SOD)水平;RT-PCR测主动脉肿瘤坏死因子(TNF)-αm RNA表达。结果模型组较对照组IL-1、MDA、TNF-αm RNA升高,IL-10、SOD降低(P<0.01);荷丹片低剂量组、荷丹片高剂量组、辛伐他汀组较模型组IL-1、MDA、TNF-αm RNA降低,IL-10、SOD升高(P<0.01)。结论荷丹片可通过抗炎、抗氧化应激作用干预AS的发生、发展。Objective To observe the effects of Hedan tablet on cytokines and oxidation factors in APOE-/-mouse, and to explore its effect on atherosclerosis and to explore its behind mechanism. Methods APOE-/-mice （n=50） were randomly divided into control group, model group, low dose Hedan tablet treatment group, high dose Hedan tablet treatment group and simvastatin treatment group. Mice in control group were given normal feed while mice in other groups were fed with high cho-lesterol diet. Hedan or Simvastatin was administrated intra-gastrically while normal saline was given to model group in the same route. After 12 weeks, mice were sacrificed to observe the mRNA level of tumor necrosis factor-α（TNF-αmRNA） in aorta by RT-PCR. Mean while, serum levels of interleukin-1 （IL-1）, interleukin-10 （IL-10）, malonaldehyde （MDA） and su-peroxide dismutase （SOD） were determined in different groups. Results Compared with control group, TNF-αmRNA tran-scription level as well as serum levels of IL-1 and MDA significantly increase while serum levels of IL-10 and SOD de-creased remarkably in model group, （P〈0.01）. Compared with model group, mRNA levels of TNF-αas well as serum levels of IL-1 and MDA were significantly decreased while serum levels of IL-10, SOD were greatly increased in low dose and high dose Hedan tablet treatment groups as well as in simvastatin treatment group （P〈0.01）. Conclusion Hedan tablet inhibit the formation of atherosclerosis through its anti-oxidation role and anti-inflammation role.

关 键 词：动脉粥样硬化 载脂蛋白E类 模型 动物 荷丹片 APOE-/-小鼠 炎症因子 氧化应激因子 

分 类 号：R972.6[医药卫生—药品] R394.114[医药卫生—药学]