大鼠急性心肌梗死后期血清代谢轮廓研究  

Investigation of serum metabolic profiling in late-stage of acute myocardium infarction in rats

作　　者：董志欢 李彤[2] 张磊[2] 杨帆[1] 

机构地区：[1]天津医科大学三中心临床学院,300170 [2]天津市第三中心医院

出　　处：《天津医药》2015年第10期1166-1170,共5页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(10JCYBJC14000)

摘　　要：目的建立急性心肌梗死(AMI)大鼠血清代谢轮廓模型,探寻对AMI预后及并发症具有临床治疗价值的特征代谢物。方法选取健康雄性Wistar大鼠20只,随机平均分为对照组和AMI 1周组。AMI 1周组进行冠状动脉前降支结扎,建立AMI模型,1周后心脏取血处死。利用代谢组学研究平台对大鼠的血液样本进行代谢轮廓分析,建立大鼠AMI后血液的正交偏最小二乘判别模型及主成分分析模型,结合SPSS 19.0软件的数据处理最终得到特征代谢产物。结果成功建立了主成分分析模型及正交偏最小二乘判别模型,同时从模型中筛选并鉴定了27种在AMI 1周组与对照组具有差异的特征代谢物,在这27种特征代谢物中溶血磷脂酰胆碱、溶血磷脂酰乙醇胺等差异明显。结论构建的代谢轮廓模型很好地拟合了AMI后期大鼠机体的代谢紊乱状态,筛选出的特征代谢产物可为该疾病治疗以及并发症的预防等提供参考和帮助。Objective A serum metabolic profiling in acute myocardial infarction （AMI） rat was established to screen potential metabolic markers of AMI prognosis and complication. Methods Male Wistar rats（n=20）were divided randomly into AMI 1 week group and control group. Anterior descending coronary was ligated in rats in AMI 1 week group to establish AMI model. After one week, these rats were sacrificed and the blood was collected from heart. And metabolomics platform was used to analyze serum metabolic profile. After PCA （principal component analysis） and OPLS-DA （Orthogonal partial least squares-Discriminant Analysis） were established, SPSS 19.0 was used to analysis data to get the potential metabolic markers. Results The PCA and OPLS-DA were established successfully and 27 metabolites present differently in levels between AMI 1 week group and the control group. Among these metabolites, LysoPC （lysophosphatidylcholine） and LysoPE （lysophosphatidyl ethanolamine） demonstrate significant differeance between these two groups. Conclusion The metabolic disorder in AMI patients can be reflected from the serum metabolic profiling. And these significant metabolites provide sup-port and reference for the prevention of AMI complication and its treatment.

关 键 词：心肌梗死 大鼠 WISTAR 色谱法 高压液相 质谱分析法 血清代谢组学 

分 类 号：R541.4[医药卫生—心血管疾病]